多氯联苯对大鼠肝脏结构及功能的影响

背景与目的： 探讨多氯联苯(polychlorinated biphenyl, PCB)对大鼠肝脏结构及功能的影响。 材料与方法： 选用40只雄性Wistar大鼠随机分为对照组(饲喂正常饲料)、3个不同PCB浓度的实验组(分别饲喂含PCB 10－8 mol/L、10－7 mol/L、10－6 mol/L饲料)，共4组。各组按上述不同的饲料连续饲喂3个月后建立慢性PCB毒性动物模型，运用光镜、免疫组化及肝生化检测技术研究PCB对大鼠肝结构及功能的影响。 结果： PCB对大鼠肝组织结构的损伤与PCB呈剂量依赖性，PCB高剂量组(10－6 mol/L)肝结构受损严重；PCB能诱导肝细胞c-fos表达， 10－7 mol/L、10－6 mol/L组肝细胞c-fos的表达明显高于对照组(P<0.05)；同时，PCB能促进肝细胞bcl-2的表达，对照组bcl-2为弱阳性表达，10－7 mol/L、10－6 mol/L组肝细胞bcl-2的表达明显高于对照组；PCB能致肝组织生化功能改变，使血中 URIC、TBLL、DBLL、AST、ALT、TP3、LDH增加，其中PCB 10－7 mol/L、10－6 mol/L剂量组上述肝脏生化指标明显高于对照组(P<0.05)。 结论： PCB对大鼠肝脏结构及功能产生明显的损伤作用。

Effects of Polychlorinated Biphenyl on Structure and Function of Rat Liver

BACKGROUND AND AIM: To study the effects of polychlorinated biphenyl(PCB) on rat liver structure and function. MATERIALS AND METHODS: 40 male Wistar rats were divided into four groups , A group was control group , B group (fed with 10-8 mol/L PCB), C group(10-7 mol/L PCB), D group(10-6 mol/L PCB) as experimental groups.After three months, all rats were killed . Immunohistochemical and biochemical methods were used to observe the effects of PCB on liver structure and function in rats. RESULTS: The strutural damage of the liver was related to the dose of PCB, with D group showing more severe damage than that of others. PCB could induce c-fos expression in the liver, which was higher in C and D groups compared with other groups (P<0.05). PCB could promote the expression of bcl-2 in liver,with higher expression in C and D groups than that of A and B groups. PCB also could affect biochemical function of liver, such as URIC,TBLL,DBLL,AST,ALT,TP3,LDH to increase in C and D groups compared with A and B groups. CONCLUSION: PCB could lead to liver injuries in the rats.