黄芪六一汤对2型糖尿病治疗效果的转录组学研究

该实验采用Illumina测序平台对大鼠胰腺进行转录组测序,对靶点进行表达量统计,分析空白组、模型组、黄芪六一汤组三者之间的表达差异关系,探究黄芪六一汤对2型糖尿病的治疗效果,并探讨其作用机制。结果表明,通过eggNOG对基因进行分类分析,其中有24.25%的基因属未知功能类,为最大分类单元,其余依次为能量转换类、氨基酸转运和代谢类、核苷酸转运和代谢类、碳水化合物转运和代谢类、辅酶转运和代谢类、脂质转运和代谢类等;根据KEGG富集分析,黄芪六一汤可能在环境信息处理、细胞过程、生物系统、人类疾病这4类代谢通路上对2型糖尿病起到治疗作用。研究表明,黄芪六一汤能显著改善2型糖尿病大鼠空腹血糖、糖化血红蛋白。

Transcriptomics of therapeutic effect of Huangqi Liuyi decoction in treating type 2 diabetes

In this study, Illumina sequencing platform was applied in sequencing rat pancreas, counting expression of target points, analyzing expression differences among blank group, model group and Huangqi Liuyi decoction group and exploring the therapeutic effect and mechanism of Huangqi Liuyi decoction on type 2 diabetes mellitus. According to the result, 24.25% of these genes belonged to the unknown functional class, which was the largest classification unit according to the classification analysis of genes by eggNOG. The rest were classified as energy conversion, amino acid transport and metabolism, nucleotide transport and metabolism, carbohydrate transport and metabolism, coenzyme transport and metabolism, and lipid transport and metabolism, etc.Huangqi Liuyi decoction may play a therapeutic role in the treatment of type 2 diabetes mellitus through four metabolic pathways, namely environmental information processing, cellular process, organismal system and human diseases according to KEGG enrichment analysis. This study shows that, Huangqi Liuyi decoction can significantly improve the fasting blood glucose and glycosylated hemoglobin in type 2 diabetic rats.