EGFL7基因沉默对裸鼠乳腺癌血管生成的影响

Objective To investigate the effect of short hairpin RNA (shRNA) targeting at epidermal growth factor-like domain 7 (EGFL7) gene on angiogenesis of breast carcinoma and its mechanism in nude mice. Methods shRNA targeting at EGFL7 gene was constructed and transfected into SGC-7901 cells (pshEGFL7 group) , meanwhile , the cells transfected with vector plasmids were as a control group. Positive clones were selected and the transplanted tumor animal models constructed in nude mice , and the growth and volume of tumors were observed. After 8 weeks , EGFL7 mRNA and protein in transplanted tumor tissues were detected,and graded. Moreover, Anti-CD34, VEGF and TSP1 were stained by the immuno- chemistry method,and MMP-2 and TIMP2 mRNA were detected by RT-PCR. Results EGFL7 mRNA was down regulated significantly in the pshEGFL7 group. In the psh EGFL7 group, the tumor volume was ( 1.86 ± 0. 65) cm3, MVD was 20. 84 ± 6.38; while in the control group , tumor volume was (4.86 ± 1.15) cm3, MVD was 39.48 ± 9.01, In the EGFL7 group ,TSP1 protein presented positive , and VEGF protein presented weakly positive or negative.The expression of MMP-2 mRNA decreased ,TIMP2 mRNA increased in the pshEGFL7 group,and there were significant differences compared with the control group , P ＜ 0.01. Conclusion RNA interference targeting at EGFL7 gene can balance TSP1/VEGF, through regulating the expression of MMP-2/TIMP2 to impair angiogenesis of breast cancer in nude mice.

Effect of RNA interference targeting at EGFL7 on angiogenesis of breast carcinoma in nude mice