结核分枝杆菌利福平耐药基因的检测

以药物敏感试验为标准，应用聚合酶链反应－单链构象多态性分析方法对６８例利福平耐药菌株和２０例利福平敏感菌株进行测定，结果８８例结核分枝杆菌均扩增出目标ＤＮＡ条带，１０例非结核分枝杆菌和８例非分枝杆菌未出现目标ＤＮＡ条带，所用引物扩增ｒｐｏＢ基因２１５ｂｐ片段为结核分枝杆菌复合群异性的。

Genetic detection of Rifampin resistant in Mycobacterium tuberculosis

Eighty eight strains of clinical isolated Mycobacterium tuberculosis were analysed by PCR SSCP,with the drug susceptibility test as contral group.The objective DNA fragments were occured in eighty eight strains of clinical isolated Mycobacterium tuberculosis, but it not occured in ten strains of Mycobacterium non tuberculosis and in eight strains of non Mycobacterium. The primer used for amplify the 215bp of rpoB gene are specificity for Mycobacterium tuberculosis complex. Among the sixty eight rifampin resistant isolates,characterization of SSCP in sixty isolates of Mycobacterium tuberculosis are different from that in standard strain of M.tuberculosis. There are no difference between the twenty susceptible strains and the standard strain.In compare with the conventional susceptibility testing methods,the sensitivity and specificity of PCR SSCP for detection of rifampin resistance in sixty eight strains of M.tuberculosis was 88% and 100%,respectively.Using specific primer to amplify the fragment of rpoB,it can not only confirm the pathogen of M.tuberculosis, but can easily and rapidly detect rifampin resistance of M.tuberculosis by the PCR SSCP technique.