大鼠闭合性硬膜外球囊扩张脑损伤膜型的建立

为建立闭合性脑硬膜外占位性颅禽损伤的大鼠模型，利用５Ｆ漂浮导管置脑硬膜外模拟占位，按球囊注入水量和持续时间分组。观察各组动物的死亡率，存活鼠的颅内压、脑含水量的变化以及血肿形成、组织受损的情况。结果显示：球囊越大，持续时间越长，损伤越大；存活鼠颅内压及脑水含量均增加，神经机能受损，并有血肿形成和受压区坏死；上述变化随占位时间延长和受压程度的加重而加重。提示该脑损伤模型简单可行、可重复，能较好地反映

A model of closed epidural balloon inflation brain injury in rats]

OBJECTIVES: To establish an animal model of closed brain epidural occupancy trauma in rats. METHODS: Sixty-eight Wistar rats were randomly divided into 7 groups: normal control group(Group A), sham-operated group(Group B), and 5 experimental groups. The last ones included: Group C in which water was injected 0.05 ml into balloon and kept for 1 hour; Group D injected 0.05 ml and kept for 3 hours; Group E injected 0.1 ml and kept for 1 hour; Group F injected 0.1 ml and kept for 2 hours. Group B, C, D were kept maintaining and observing for two days after operation, respectively. Group E was divided into EI (maintaining for two days) and EII (for three days). RESULTS: Of all the experimental groups, the mortality rate during acute phase was 100% in Group F, 28% in Group E, 50% in Group D, and 0% in Group C. Four rats died during the raising-up period in Group E. Both ICP and cerebral water content were increased in 46 survived rats, and nerve functions were damaged in all the experimental groups. Pathologic changes read as follows: Hemorrhage was found in damage-area; injury and dead cells were seen in compressed region. These changes became worse with time prolonged and injury degree developed. CONCLUSIONS: The method is simple, reproducible and economical, well showing the pathological classification of epidural occupancy trauma.