长沙地区小儿载脂蛋白E基因多态性及其对血脂的影响

采用改良的ＰＣＲ－ＲＦＬＰ方法测定长沙地区２９１名儿童载脂蛋白（Ａｐｏ）Ｅ基因型。结果发现，ε２，ε３，ε４等位基因的频率分别是０．０８１，０．８５７和０．０６２。ＡｐｏＥ等位基因频率分布无明显的性别差异。Ａｐｏε２等位基因携带得与其它ＡｐｏＥ基因携带者相比，血高密度脂蛋白－胆固醇、ＡｐｏＥ和ＡｐｏＡ１浓度较高，而总胆固醇、低密度脂蛋白－胆固醇、ＡｐｏＢ１００水平较低，ε４等位基因推和的红脂、脂蛋

Apolipoprotein E polymorphism and plasma lipid, lipoprotein, apolipoprotein levels in 291 children of Changsha]

In order to investigate the distribution of apolipoprotein (Apo) E polymorphism and effect of ApoE genotypes on plasma lipid, lipoprotein, apolipoprotein levels in children of Changsha, we determined ApoE genotypes in 291 children by means of PCR-RFLP. ApoE allele frequencies in the population were epsilon 2 = 0.081, epsilon 3 = 0.857, epsilon 4 = 0.062. There were no difference in the ApoE genotypes distribution between sexes. The children with Apo epsilon 2 allele had higher levels of ApoE, ApoA1 and high density lipoprotein-cholesterol together with lower levels of total cholesterol, ApoB100 and low density lipoprotein-cholesterol than those with other ApoE alleles. These changes were opposite in children with Apo epsilon 4 allele. The levels of plasma triglyceride, lipoprotein(a) were not affected significantly by ApoE alleles. Moreover, the effect of ApoE genotypes on levels of plasma lipids was different between sexes. These results suggest that ApoE polymorphisms could affect plasma lipids metabolism in children.