| 高效液相色谱法测定全血环孢霉素A |
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| 引用本文： | 曾立明 黄宇丹. 高效液相色谱法测定全血环孢霉素A[J]. 湖南医科大学学报, 1999, 24(4): 387-389 |
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| 作者姓名： | 曾立明 黄宇丹 |
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| 摘 要： | 采用Ｃ１８反相色谱柱法测定定值全血（Ｂ１０－ＲＡＤ），３个浓度的水平。结果均在预期范围内，其最低检出浓度为２５ｍｇ．ｍｌ＾－１，到嫠在５０～１００ｍｇ．ｍｌ＾－１范围内呈线性，绝对回收率为９９．６％～１０８．８％；批内和批间是系数分别为７．２％和９．３％。
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| 关 键 词： | 环孢霉素A 血药浓度 高效液相色谱法 |
Measurement of cyclosporine A in whole blood by high performance liquid chromatography(HPLC)] |
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| L Zeng,Y Huang,G Peng. Measurement of cyclosporine A in whole blood by high performance liquid chromatography(HPLC)][J]. Bulletin of Hunan Medical University, 1999, 24(4): 387-389 |
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| Authors: | L Zeng Y Huang G Peng |
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| Affiliation: | Department of Clinical Laboratory, Xiangya Hospital, Hunan Medical University, Changsha 410008. |
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| Abstract: | Whole blood was extracted by ether under base using cyclosporine D as internal standard. The extraction was evaporated and reconstituted. The interferences were further removed by hexane. The reconstitution was injected into a C18 reversed-phase column at 70 degrees C, which was eluted with a mobile phase of acetonitrite/methanol/water(60/10/30, v/v) at flow rate 1.6 ml.min-1 and monitored at 210 nm. Three levels of BIO-RAD cyclosporine A(whole blood) calibrator were measured, all the results were within expected range. The detection limit was 25 ng.ml-1. The linearity was at least within 50-1,000 ng.ml-1. Analytical recoveries were 99.6%-108.8%. The within-run cv and between-run CV were 7.2% and 9.3%, respectively. |
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