| 福尔马林固定组织电镜标本制作法 |
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| 引用本文： | 井建华. 福尔马林固定组织电镜标本制作法[J]. 湖南医科大学学报, 1999, 24(6): 581-582 |
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| 作者姓名： | 井建华 |
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| 摘 要： | 本文介绍了一种能使福尔马林固定、石蜡包埋的组织达到电镜观察要求的样品制备方法。对于取材新鲜、固定及时的石蜡包埋组织，脱蜡后再以４％戊二醛固定２４ｈ，１％锇酸后固定１￣２ｈ，再常规丙酮脱水、Ｅｐｏｎ８１２包埋、超薄切片，可获得基本上符合诊断要求的超微结构。此外，本文还对该方法的要点及注意事项进行了讨论。
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| 关 键 词： | 电子显微镜 福尔马林 石蜡包埋 |
Sample preparation for electron microscope with tissues fixed with formalin and embedded in paraffin] |
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| J Jing. Sample preparation for electron microscope with tissues fixed with formalin and embedded in paraffin][J]. Bulletin of Hunan Medical University, 1999, 24(6): 581-582 |
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| Authors: | J Jing |
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| Affiliation: | Electron Microscope Laboratory, Hunan Medical University, Changsha 410078. |
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| Abstract: | A method of sample preparation for electron microscope is introduced. After paraffin-embedded tissues, fresh and fixed on time, were deparaffined, the tissue were fixed in 4% glutaraldehyde for 24 hours, in 1% osmic acid for 1-2 hours, and dehydrated with acetone and embedded in Epon812; then they were cut into ultrathin sections. By this method, better ultrastructure of paraffin-embedded tissues was obtained, and the method could satisfy the demand for observation under electron microscope. In addition, some important proceedings were also discussed in the paper. |
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