| 单克隆抗体ELISA测定血浆富组氨酸糖蛋白的方法 |
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| 引用本文： | 徐克前,陈正炎.单克隆抗体ELISA测定血浆富组氨酸糖蛋白的方法[J].湖南医科大学学报,1999,24(3):229-231. |
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| 作者姓名： | 徐克前 陈正炎 |
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| 摘 要： | 建立用单克隆抗体酶联免疫吸附测定法（ＥＬＩＳＡ）测定血浆富组氨酸糖蛋白（ＨＲＧ）的方法。结果显示：ＭｃＡｂ３Ｃ６Ｆ３包被浓度为１０μｇ·ｍｌ＾－１，单抗酶标结合物作１：２００稀释时标准曲线最理想；最低检出限为１ｎｇ·ｍｌ＾－１，批内ＣＶ８．２％，批间ＣＶ１１．５％，回收率为８９．１％，测定范围为２．５－８０ｎｇ·ｍｌ＾－１；用该法测定４０例正常人血浆ＨＲＧ为（１１０．３±９．７）ｍｇ·Ｌ＾－１，２
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| 关 键 词： | 富组氨酸糖蛋白 血浆 单克隆抗体 ELISA |
Establishment of sandwich ELISA with McAbs to measure histidine-rich glycoprotein in plasma] |
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| K Xu,Z Chen.Establishment of sandwich ELISA with McAbs to measure histidine-rich glycoprotein in plasma][J].Bulletin of Hunan Medical University,1999,24(3):229-231. |
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| Authors: | K Xu Z Chen |
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| Institution: | Department of Clinical Biochemistry, Hunan Medical University, Changsha 410013. |
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| Abstract: | The objective of this paper is to establish a method using sandwich ELISA with McAbs to measure plasma histidine-rich glycoprotein (HRG). The results showed that this method, with a working range from 2.5 ng.ml-1 to 80 ng.ml-1, provided the lower limit of detection of 1 ng.ml-1, the average intra-assay CV, the interassay CV and the recovery rate of HRG were 8.2%, 11.5% and 89.1%, respectively. Interference by antithrombin III and human albumin was not significant. With this method, the concentration of plasma HRG from healthy donors was (110.3 +/- 9.7) mg.L-1, and the value of plasma HRG in liver cirrhosis was (79.5 +/- 12.6) mg.L-1. These results suggest that sandwich ELISA with McAbs is high sensitive, precise and specific. |
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