鼻咽癌细胞凋亡与细胞增殖的原位观察

Observation of apoptosis and proliferation in nasopharyngeal carcinoma]

In order to observe the apoptosis and proliferation of cells in nasopharyngeal carcinoma(NPC) and pericarcinomatous tissue(PCT), 49 cases of NPC and partly PCT were detected by in situ end-labelling (ISEL) technique and proliferating cell nuclear antigen(PCNA) immunohistochemical staining. The results showed that: 1. Massive distribution of ISEL positive cells was frequently exhibited in vesicular nucleus cell carcinoma(VNCC) of NPC, in which no apoptotic pattern character was found. 2. The positive rate and the staining intensity index(SII) of ISEL signals in NPC were higher than that in PCT(P < 0.05); the positive correlation between ISEL SII and PCNA SII was found in NPC(r = 0.341, P < 0.05), whereas the positive rate and the SII of ISEL signals were significantly lower than that of PCNA (P < 0.01). 3. The ISEL SII and the PCNA SII in VNCC were obviously higher than that in poorly differentiated squamous cell carcinoma(PDSCC) (P < 0.01, P < 0.05). Patients with VNCC had a high five-year survival rate. The results suggest that there is a relationship between cellular proliferation and cellular apoptosis in NPC.