| 重组日本血吸虫SjGT—Sj32蛋白诱导小鼠保护免疫的研究 |
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| 引用本文： | 蔡春,易新元.重组日本血吸虫SjGT—Sj32蛋白诱导小鼠保护免疫的研究[J].湖南医科大学学报,1999,24(3):225-228. |
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| 作者姓名： | 蔡春 易新元 |
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| 摘 要： | 应用基因工程技术分别表达出ＳｊＧＳＴ－Ｓｊ３２，Ｓｊ３２和ＳｊＧＳＴ，用于保护性免疫的动物实验及其体液免疫机制的初步探讨。结果：与对照组相比，重组ＳｊＧＳＴ－Ｓｊ３２组，Ｓｊ３２组，Ｓｊ３２＋ＳｊＧＳＴ组均有明显的减虫效果。上述三组及ＳｊＧＳＴ组的肝脏虫卵计数的均较对照组为少。提示重组Ｓｊ３２ｋＤ蛋白可诱导小鼠产生明显的抗日本血吸虫攻击感染的保护性免疫及抗生殖免疫效果，而ＳｊＧＳＴ仅表现出一定的抗
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| 关 键 词： | 日本血吸虫 重组蛋白类 疫苗 免疫 小鼠 |
Schistosoma japonicum: protective immunity induced in mice immunized with SjGST-Sj32] |
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| C Cai,X Yi,X Zeng,J Zhou,X Shu.Schistosoma japonicum: protective immunity induced in mice immunized with SjGST-Sj32][J].Bulletin of Hunan Medical University,1999,24(3):225-228. |
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| Authors: | C Cai X Yi X Zeng J Zhou X Shu |
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| Institution: | Department of Parasitology, Hunan Medical University, Changsha 410078. |
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| Abstract: | Schistosoma japonicum glutathione-s-transferase (SjGST), Schistosoma japonicum 32,000 protein (Sj32) and SjGST - Sj32 were expressed and purified to study on protective immunity against Schistosoma japonicum. The result showed that compared with the adjuvant control group, the worm burden was decreased significantly in mice immunized with recombinant SjGST - Sj32, Sj32 and Sj32 + SjGST (P < 0.05). At the same time, the liver egg per gram(EPG) was decreased in mice immunized with SjGST - Sj32, Sj32, Sj32 + SjGST and SjGST. It is concluded that Sj32 significantly induces both protective immunity and anti-fecundity immunity, but SjGST only has an anti-fecundity effect. |
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