N－糖苷键型糖蛋白与肺癌诊断的初步研究

为探讨Ｎ－糖苷键型糖蛋白在肺癌诊断中的意义。取肺鳞癌、腺癌、大细胞癌、小细胞癌术后切除新鲜癌组织匀浆混匀，提取蛋白质并标记上辣根过氧化物酶，在ＥＬＩSＡ板上与血清中Ｎ－糖苷键型糖蛋白反应，按常规方法显色。以正常人血清与肺癌蛋白提取物反应，在４９０ｎｍ的吸光度平均值加两个标准差作为阳性阈值。肺癌病人血清的阳性率４３．３％（２６／６０），非癌性肺病为１５．０％（６／４０），健康对照组为１０．０％（４／４０），肺癌病人血清的阳性率与病理类型无相关性。结果提示，肺癌病人血清中Ｎ－糖苷键型糖蛋白能较特异地与肺癌组织提取蛋白相结合。

Evaluation of the significance of N-linked glycoproteins in sera in thediagnosis of lung cancer patients

In order to evaluate the significance of N- linked glycoproteins in sera in the diagnosis of lungcancer patients, serum samples were collected from 60 patients vvith lung cancer, 40 patients with benignpulmonary disease and 40 normal volunteers.Tumor proteins were extracted from the mixture of neo-plasms of several patients,The tumor protein labled with HRP were used as a probe in ELISA against N-linked glycoproteins in sera. The result was assayed at 490nm with ELISA detector,the mean value of ab-sorbance of normal volunteers plus two standard deviations was used as the positive threshold. The positiverection rate of tumor protein extracts and N-linked glycoproteins was 43.3%(26/40)in patients with lungcancer,15%(6/40)in patients with benign resparitory disease, 10.0%(4/40) in normal volunteers, Therewere no significant differences of positive rates in different pathological types of lung cancer patients. Theresults suggest that N-linked glycoproteins can react with tumor protein extracts.