单克隆抗体作单纯疱疹病毒糖蛋白的特性鉴定以及临床应用

应用本室制备的8株抗单纯疱疹病毒糖蛋白单克隆抗体(抗HSV McAb)进行了系列研究,①鉴定出一种新的HSV糖蛋白g30K、gC的一种新形式,以及gC和gD;②建立了HSV感染的临床标本作抗原抗体分型检测的McAb-ELISA双夹心法,检测961份标本效果良好,并己在全国各地一些医疗单位推广使用;③证明McAb治疗家兔实验性单纯疱疹性角膜炎(HSK)有显著效果,并探讨其治疗机理主要为中和作用和ADCC效应;④对部分志愿者用McAb治疗单纯疱疹性角膜炎、妇女生殖器疱疹和小儿口腔疱疹性糜烂,取得明显疗效。

Characterization of Herpes Simplex Virus Glycoproteins and Clinical Application of Monoclonal Antibodies to Herpes Simplex Virus

A series of research work has been completed in recent years in this labor- atory with monoclonal antibodies (McAb) against herpes simplex virus (HSV). (1) A novel HSV-2 glycoprotein (g30K) was identified and the gene coding for it was localized to the unique long (UL) region at map units 0.490 to 0.564. The target antigens of 7 McAbs (gC and gD) were also characterized. (2) Two double sandwich McAb-ELISA tests were developed to detect and type HSV antigen and antibody in more than 1000 clinical specimens. (3) The rabbits with experimental herpes simplex keratitis (HSK) by infection with HSV were treated with McAbs to HSV gC and gD. All the rabbits in McAb treat group were cured. The mechanisms of treatment are involved in neutralization and ADCC. (4) 204 patients with HSK, herpetic cervicitis, herpetic vulvitis and herpetic stomatitis received anti-HSV McAb therapy. The effect rates were 100%, 92.6%, 100% and 95.5%, respectively. The evidences suggest that McAbs may be effective agents to treat HSV infection.