魟鱼软骨多糖对Lewis肺癌MMP-9表达的影响

目的:鱼软骨多糖(RCG)对Lewis肺癌基质金属蛋白酶9(MMP-9)表达的影响。方法:建立小鼠Lewis肺癌模型,将动物分为生理盐水组、不同剂量鱼软骨多糖组、环磷酰胺组,观察肿瘤生长情况,绘制肿瘤生长曲线,计算原发瘤抑制率;采用RT-PCR和Western blot检测瘤组织MMP-9基因和蛋白表达。结果:该多糖各剂量组小鼠的肿瘤生长曲线平缓,小鼠原发瘤抑制率、肺转移数与生理盐水组比较差异有显著性(P<0.05;P<0.01);与生理盐水比较,鱼软骨多糖各剂量组,MMP-9 mRNA和蛋白表达水平显著降低(P<0.01)。结论:鱼软骨多糖明显抑制小鼠Lewis肺癌原发瘤的生长和转移,可能与其抑制肿瘤MMP-9基因和蛋白表达有关。

Effect of ray cartilage glycosaminoglycans(RCG)on the expression of MMP-9 in Lewis lung carcinoma

Objective: To explore the effect of Ray cartilage glycosaminoglycans(RCG) on the expression of MMP-9 in Lewis lung carcinoma of mice.Method: The model of mice with Lewis lung carcinoma was induced.The experimental mice were randomly divided into normal saline group,RCG groups at varied concentrations and CTX group.Tumor growth state was observed,and tumor inhibitory rate of primary tumor and number of lung metastasis focus were measured.The expression of MMP-9 mRNA and protein in Lewis lung carcinoma was determined with RT-PCR and Western blot.Result: As compared with normal saline group,tumor growth curves in RCG groups were smooth,there were significant differences of inhibitory rates of primary tumor and number of lung metastasis focus between RCG groups and normal saline group,and MMP-9 mRNA and protein expression levels in RCG groups were reduced significantly.Conclusion: RCG can inhibit effectively the growth and metastasis of implanted Lewis lung carcinoma in C57BL/6 mice,which is probably attributed to reducing the expression of MMP-9 mRNA and protein.