Induction of tolerance to ischemia: Alterations in second-messenger systems in the gerbil hippocampus |
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Authors: | Hiroyuki Kato Tsutomu Araki Kenshi Murase Kyuya Kogure |
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Institution: | Department of Neurology, Tohoku University School of Medicine, Sendai, Japan. |
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Abstract: | Preconditioning the brain with sublethal ischemia protects against neuronal damage following subsequent ischemic insult. Using 3H]inositol 1,4,5-triphosphate (IP3), 3H]phorbol 12,13-dibutyrate (PDBu), 3H]cyclic adenosine monophosphate (cAMP) and 3H]rolipram, we performed quantitative autoradiography to determine postischemic alterations in second-messenger systems in the gerbil hippocampus following preconditioning the brain with sublethal ischemia. At 7 days of reperfusion, no alterations were observed in brains subjected to 2 min of forebrain ischemia which produced no neuronal damage. However, 3-min ischemia caused a 75% reduction in 3H]IP3 binding (p < 0.01 vs. control) and 15-25% reductions in 3H]forskolin (p < 0.01 vs. control), 3H]cAMP (p < 0.05 vs. control), and 3H]rolipram (p < 0.01 vs. control) binding in the CA1 subfield coincident with histopathological CA1 pyramidal cell destruction, but no significant alterations in 3H]PDBu binding. Preconditioning the brain with 2 min of ischemia followed by 4 days of reperfusion prevented both histopathological cell death and the reductions in binding following subsequent 3 min of ischemia. Interestingly, 3H]IP3 and 3H]rolipram binding in CA1 showed a transient reduction, by 30% and 20% (both p < 0.01 vs. control), respectively, in the early reperfusion period. This downregulation of the IP3 system may play a role in the protection against cell death. |
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Keywords: | Cerebral ischemia Hippocampus Gerbil Preconditioning Tolerance Autoradiography Second messengers Inositol triphosphate |
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