隐孢子虫病的病原诊断

在南京四所医院收集腹泻患者粪便1014人份,进行隐孢子虫感染调查。每份粪便涂片后先用金胺-酚染色法过筛检查卵囊,发现阳性或可疑标本,再用改良抗酸和沙黄-美蓝染色法检查。结果,金胺-酚染色法阳性13例,用后两种染色法都找到卵囊;可疑者5例,后两种方法卵囊均为阴性。金胺-酚和改良抗酸染色法联用时,方法简便,效果好。卵囊少时,沙黄-美蓝染色法不易发现阳性。

Etiological diagnosis of cryptosporidiosis

1,014 faecal specimens were collected from 4 hospitals in Nanjing to investigate the infection of Cryptosporidium. Each faecal specimen was smeared and screened by auramine phenol staining method for oocyst, the positive and suspected specimens were identified by modified acid-fast staining method and safranin-methylene blue staining method. The result showed that 13 specimens were positive by auramine-phenol staining method and confirmed by modified acid-fast staining method. 5 suspected specimens were negative as shown by the latter two methods. The best result could be obtained when auramine-phenol staining method was first used and followed by modified acid-fast staining method. This technique is simple, sensitive and reliable. It is difficult to find oocysts by safranin methylene blue staining method when the oocysts are few.