复发性流产患者外周血T淋巴细胞亚群及CD_4~ 辅助性T淋巴细胞亚型的变化

目的　探讨不明原因的复发性流产 (URSA)与T淋巴细胞免疫功能的关系。方法　采用酶联免疫斑点 (ELISPOT)法对 30例URSA患者和 2 4例正常妇女 (对照 )的外周血T淋巴细胞亚群及CD 4 辅助性T淋巴细胞 (Th细胞 )亚型进行检测。结果　与对照相比 ,URSA患者外周血中CD 4 辅助性T淋巴细胞百分率增多 (分别为 45 %、6 0 % ,P <0 0 5 ) ,Th1细胞的百分率升高 (14%与 2 6 % ,P<0 0 1) ,Th2细胞的百分率降低 (2 0 %与 7% ,P <0 0 5 ) ,Th1/Th2细胞比值增大 (0 73与 3 80 ,P<0 0 1)。结论　URSA的发生可能与CD 4 辅助性T淋巴细胞的增多及Th1细胞介导的细胞免疫功能的增强有关。

Changes of T Lymphocyte and CD_4~ T Helper Subsets in Peripheral Blood from Women with Unexplained Recurrent Spontaneous Abortion

Objective To investigate the relationship between occurrence of unexplained recurrent spontaneous abortion(URSA) and immune function of T lymphocytes. Methods T lymphocyte subsets and CD 4 T helper subsets (including T helper 1 and T helper 2 cells) in peripheral blood from 30 cases with URSA and 24 healthy women (controls) were detected by enzyme-linked immunospot assay (ELISPOT). Results The percentage of CD 4 positive T lymphocytes in URSA group was higher than that in the controls (60% vs 45%, P <0 05). Compared with the normal group, the percentage of Th1 cells increased (26% vs 14%, P <0 01) but the percentage of Th2 cells decreased (7% vs 20%, P <0 05), the ratio of Th1 to Th2 increased (3 80 vs 0 73, P <0 01) in the URSA group. Conclusions The occurrence of URSA might be associated with proliferation of the CD 4 positive T cells and enhancement of cellular immune function mediated by Th1 cells.