A major change in surface antigens during the maturation of newborn larvae of Trichinella spiralis |
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Authors: | Michele Jungery Nicholas W.T. Clark R.Michael Parkhouse |
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Affiliation: | 1. Tropical Disease Unit, Nuffield Department of Clinical Medicine, John Radcliffe Hospital, Oxford OX3 9DU, England;2. Division of Immunology, National Institute for Medical Research, Mill Hill, London NW7 1AA, England |
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Abstract: | Newborn larvae of Trichinella spiralis were collected for 30 min from female worms in culture, incubated in vitro for various times up to 18 h, and surface-labelled with iodine. The detergent-solubilised products were examined by SDS-polyacrylamide gel electrophoresis. At time periods up to 6 h these larvae expressed only one Mr 64 000 iodine-labelled surface protein. Some time between 6 h and 18 h a further three components (apparent Mr 58 000, 34 000 and 32 000) became accessible to surface labelling. All four of these components are antigenic in that they can be immunoprecipitated with T. spiralis immune sera. Tryptic peptide analysis revealed that the 32 and 34 kDa antigens were structurally very similar, but the 58 and 64 kDa proteins differed from each other and the 32–34 kDa pair. Thus T. spiralis not only undergoes a total change in surface antigens between moults, but also major changes in surface antigen expression within one stage. |
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Keywords: | Nematode surface antigens PBS phosphate-buffered saline FCS foetal calf serum NBL newborn larvae SDS sodium dodecyl sulphate PAGE polyacrylamide gel electrophoresis kDa kilodalton |
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