护肝合剂对慢性乙型肝炎患者PBMC功能的影响

目的观察护肝合剂对慢性乙型肝炎患者肝功能、外周血单个核细胞（PBMC）功能的影响。方法治疗组50例．在一般治疗的基础上加用护肝合剂，对照组15例用甘草酸二胺氯化钠溶液、门冬氨酸鸟氨酸、灯盏花素等进行一般治疗。分离培养治疗前后外周血单核细胞，加入植物血凝素（PHA），培养48h后，1500r／min离心10min，收集上清液-20℃保存，统一检测IFN-y，IL-10含量。采用流式细胞仪检测治疗前后外周血CD8^＋CD28^＋T细胞亚群。结果治疗组谷丙转氨酶（ALT）、谷草转氨酶（AST），黄疸指数（TBIL）复常率均明显优于对照组（P〈0．05，P〈0．01，P〈0．01）。治疗组治疗后IFN-y显著升高（P〈0．05），IL-10显著下降（P〈0．05），对照组升高不显著（P〉0．05）。CD8^＋CD28^＋T细胞亚群治疗组较对照组显著升高（P〈0．05），且治疗组治疗前后亦显著升高（P〈0．05）。结论护肝合剂能够改善肝功能，提高慢性乙型肝炎患者Th1细胞免疫。

Effect of Huganheji on PBMC Functions in Patients with Hepatitis B Virus Infection

Objective To evaluate the effect of Huganheji on improving liver functions and immunologic functions of peripheral blood mononuclear cells in patients with hepatitis B virus infection. Method 65 hepatitis B patients was divided into 2 groups. 50 patients were treated with Huganheji by oral administration. 15 patients were treated with conventional therapies. Peripheral blood mononuclear cells were collected and incubated with PHA for 48 hours. After incubation, cell free supernatant was collected and stored at -20℃ until use. Levels of IFN-γand IL-10 in the culture supernatant were determined. Flow cytometric method was used to analyze the level of CD4 and CD8 cells in peripheral blood. Result After treatment with Huganheji, the levels of ALT, AST and TBIL were restored to normal levels. The level of IFN-γ was significantly increased （P〈0.05） while IL-10 was decreased significantly （P〈 0.05）. Significant change of IFN-~ and IL-10 in the patients receiving conventional treatment was not observed （P〉 0.05）. Conclusion Huganheji can improve liver functions and increase Thl cells in hepatitis B patients.