玉屏风散对S180荷瘤小鼠Th1／Th2型细胞因子的影响

目的探讨玉屏风散对荷瘤小鼠Th1／Th2型细胞因子产生的影响。方法采用体外培养S180肉瘤细胞，接种C57BL／6纯系小鼠，建立荷瘤小鼠模型，设正常对照、荷瘤对照及玉屏风散给药组，检测各组脾脏T淋巴细胞增殖能力及Th1（IL-2、IFN-γ）和Th2（IL-4、IL-10）细胞因子产生水平。结果荷瘤组T细胞增殖能力明显下降，Th2型细胞因子IL-10的产生明显增加，与正常对照组比较均有统计学意义（P〈0．01），而IL-4的含量略有增加，但无统计学意义。Th1型细胞因子IL-2及IFN-γ的产生明显减少，与正常对照组比较有统计学意义（P〈0．01，P〈0．05）。玉屏风散给药对T细胞增殖能力及细胞因子的产生有较为明显的调节作用，与荷瘤组比较T细胞增殖能力及IL-2、IFN-γ的产生明显增加（P〈0．01），Th2型细胞因子IL-10的血清含量下降（P〈0．01）．IL-4的含量略有下降，但与荷瘤对照组比较无统计学意义。结论玉屏风散可有效调节荷瘤小鼠的免疫功能，促进荷瘤鼠Th1型细胞因子的产生，有效纠正荷瘤导致Th1／Th2的失衡，增强机体的抗肿瘤免疫功能。

Influence of Yupingfengsan on Th1/Th2 Cytokines in Mice Bearing S180 Tumor Cells

Objective To explore the influence of Yupingfengsan on the Th1 and Th2 cytokines in tumor-bearing mice. Method Tumor-bearing mice were established by inoculated with S180 cell line. Mice were divided into normal control group, tumor-bearing control group and Yupingfengsan group （tumor-bearing mice treated with Yupingfengsan for 12 days）. 12 days later, spleen cells were collected for the determination of T cell proliferation and the expression of Th1 cytokines （IL-2, IFN-γ） and Th2 cytokines （IL-4, IL-10）. Result Compared with normal control, T lymphocyte proliferation and Th1 cytokines （IL-2, IFN-γ） decreased and Th2 cytokine IL-10 increased （P 〈0.01 ） in tumor-bearing mice, but IL-4 had no difference between the two groups. Compared with tumor-bearing mice, T lymphocyte proliferation and Th1 cytokines （IL-2, IFN-γ） increased and Th2 cytokine IL-10 decreased （P〈 0.01）in the mice of Yupingfengsan group, but IL-4 had no difference between the two groups. Conclusion Yupingfengsan can promote the secretion of Th1 and correct Th1/Th2 disequilibrium resulted from tumor-bearing.