深圳地区人群2型糖尿病相关基因多态性的研究 |
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引用本文: | 高玲,;刘茂玲,;吴少庭,;钟严伟. 深圳地区人群2型糖尿病相关基因多态性的研究[J]. 广东寄生虫学会年报, 2008, 0(6): 553-555 |
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作者姓名: | 高玲, 刘茂玲, 吴少庭, 钟严伟 |
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作者单位: | [1]深圳市平湖预防保健所,深圳518111; [2]深圳市疾病预防控制中心,深圳518020 |
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摘 要: | 目的研究分析深圳地区2型糖尿病(DM)相关基因浆细胞膜糖蛋白(PC-1)基因K121Q多态性,探讨其于2型DM的关系。方法选择214例2型DM患者和295例非DM对照人群作病例对照研究。聚合酶链反应-限制性酶切片段长度多态性(PCR-RFLP)检测PC-1基因K121Q多态性。并分析其与2型DM的相关性。结果PC-1基因PCR扩增片段大小为240bp,与预期的扩增片段大小相符。扩增片段经限制性内切酶AvaⅡ酶切后,产生Q与K两个等位基因,出现2种基因型条带,分别为KK型和QK型。509例研究对象中,KK基因型者433例,所占比例最高,为85.07%;KQ基因型者次之,有76例,占14.93%;未见QQ基因型。K等位基因频率为92.53%,Q等位基因频率为7.47%。各组基因型频率及等位基因频率比较,差异均有统计学意义(P〈0.05)。以是否患2型糖尿病为因变量,PC.1基因K121Q基因型为自变量,进行Logistic回归分析。单因素分析结果表明,与KK基因型者相比,KQ基因型与2型DM存在显著相关性,其P=0.034,OR=1.84,OR95%CI为(1.05-3.24)。结论PC-1基因K121Q多态性与2型糖尿病存在相关性,KQ基因型可能是2型糖尿病的危险因素之一。
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关 键 词: | 膜糖蛋白 基因多态性 2型糖尿病 |
Studies on the Polymorphism of PC-Gene and Its Relationship with Type 2 Diabetes Mellitus among Chinese Population in Shenzhen Areas |
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Affiliation: | GAO Ling, LIU Mao-ling, WU Shao-ting, ZHONG Yan-wei (1.PinHu Prevention and Health Institute of Shenzhen, Shenzhen 518111; 2. Shenzhen Center for Disease Control and Prevention, Shenzhen 518020, China) |
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Abstract: | Objective To investigate the K121Q polymorphism of membrane glycoprotein PC-1 gene, and its relationship with type 2 diabetes mellitus. Method Case control study was carried out among 509 subjects, including 214 patients with type 2 diabetes mellits and 295 controls with normal glucose tolerance. Genotypes of K121Q were determined by polymerase chain reaction-restriction fragment length polymorphisms (PCR-RFLP) and DNA sequencing. Result The frequencies of genotype in the patients and control groups were 80.68% and 91.12% for KK, and 19.32% and 8.88% for KQ. The genotype of QQ was not found. The allele frequencies in the patients and control group were 90.34% and 95.56% for K, and 9.66% and 4.44% for Q. The difference in the genotype frequency and allele frequency between the two groups was significant (P〈0.05). Results from the logistic regression analysis indicated that the occurrence of type 2 diabetes mellitus wasassociated with the KQ genotype (P=0.034, OR=1.84,OR95% CI=1.05-3.24). Conclusion The K121Q polymorphism of PC-1 gene was correlated with type 2 diabetes mellitus. KQ genotype may be one of the risk factors. |
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Keywords: | membrane glycoprotein gene polymorphism 2 type diabetes mellitus |
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