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Sephadex—G50微柱凝胶血型试剂的研制和应用
引用本文:罗广平,叶欣,戎霞,汪传喜,夏文杰,陈扬凯,邓晶,赵阳,丁浩强. Sephadex—G50微柱凝胶血型试剂的研制和应用[J]. 广东寄生虫学会年报, 2008, 0(7): 657-659
作者姓名:罗广平  叶欣  戎霞  汪传喜  夏文杰  陈扬凯  邓晶  赵阳  丁浩强
作者单位:广州血液中心,广州510095
基金项目:广州市医药卫生科技项目(No.2005ZDi09).
摘    要:目的 研制卡式微柱凝胶血型试剂并应用于血型血清学试验。方法 以Sephadex-G50超细凝胶为分子筛载体,制备成中性微柱凝胶试剂卡、特异性凝胶试剂卡及coomb’s凝胶试剂卡并用于血型鉴定、配血试验、抗体筛选和鉴定,对1000名献血者ABO及Rh(D)血型进行鉴定,其中A型272例、B型279例、B3亚型1例、0型437例、AB型11例;Rh(D)阴性2例,Rh(D)阳性998例;对100例输血前检查标本进行配血试验;以Coomb's凝胶试剂卡对抗-D、-C、-c、-E、-e、-Jk^a、-Jk^b、-Fy^a、-Di^a等9个抗体分别与谱红细胞反应做特异性抗体鉴定试验:同时以瑞士进口的DiaMed同类产品及常规的血型血清学试管法同步比较。结果 研制的卡式微柱凝胶血型试剂与瑞士进口的DiaMed同类产品比较,检验结果、敏感性、特异性无明显差别。结论 研制的卡式微柱凝胶试剂适用于血型鉴定、配血试验、抗体筛选和鉴定等血型血清学检查,该试剂制备具有成本低、工艺简单的优点,具有较好的推广应用和开发价值。

关 键 词:微柱凝胶  血型血清学检测  Sephadex-G50

Development of Sephadex-G50 Microcolumn Technique for Blood Group Serological Detection
LUO Guang-ping,YE Xin,RONG Xia,WANG Chuan-xi,XIA Wen-jie,CHEN Yang-kai,DENG Jing,ZHAO Yang,DING Hao-qiang. Development of Sephadex-G50 Microcolumn Technique for Blood Group Serological Detection[J]. Journal of Tropical Medicine, 2008, 0(7): 657-659
Authors:LUO Guang-ping  YE Xin  RONG Xia  WANG Chuan-xi  XIA Wen-jie  CHEN Yang-kai  DENG Jing  ZHAO Yang  DING Hao-qiang
Affiliation:( Guangzhou Blood Center, Guangzhou 510095,China)
Abstract:Objective To study the application of sephadex-G50 microcolumn technique established by Guangzhou Blood Center for blood group serology. Methods Sephadex-G50 microcolumn was developed by our center for blood group typing. The blood samples of 1 000 blood donors were identified by routine typing, the sephadex-G50 microcolumn and the microcolumn gel (DiaMed, Switzerland). 100 cross-matching of blood samples were detected by the three methods. The special antibodies, such as -D,-C, -c, -E, -e, -Jk^a, -Jk^b, -Fy^a, -Dia were detected by the three methods. Results There were no difference between the sephadex-G50 microcolumn and the microcolumn gel (DiaMed, Switzerland). Conclusion Our sephadex-G50 microcolumn is as good as the microcolumn gel (DiaMed, Switzerland), but cheaper. It is suitable for blood group typing.
Keywords:microcolnmn  blood group serological detecting  sephadex-G50
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