| 紫外-可见分光光度法测定紫花高乌头中乌头碱的含量 |
| |
| 引用本文： | 任文栓,马佳,常福厚.紫外-可见分光光度法测定紫花高乌头中乌头碱的含量[J].内蒙古医学杂志,2010,42(2):136-139. |
| |
| 作者姓名： | 任文栓 马佳 常福厚 |
| |
| 作者单位： | 1. 内蒙古自治区医院医务科,内蒙古,呼和浩特,010017
2. 内蒙古医学院药学院,内蒙古,呼和浩特,010059 |
| |
| 基金项目： | 内蒙古医学院蒙药现代化研究重大项目资助(MX2005ZB010) |
| |
| 摘 要： | 目的：测定紫花高乌头中乌头碱的含量,为蒙药紫花高乌头中乌头碱的含量的测定提供方法,制定质控标准。方法：采用紫外-可见分光光度法,在520 nm的波长处测定吸光度,从标准曲线上读出供试品溶液中酯型生物碱的重量。结果：乌头碱浓度在15.1-75.5μg/ml范围内呈良好的线性关系。回归方程为Y=0.0086 X＋0.13,r=0.9993,本品每克含酯型生物碱不得超过5 mg。结论：采用紫外-可见分光光度法测定紫花高乌头中乌头碱的含量,具有操作简便、结果准确的特点。
|
| 关 键 词： | 紫花高乌头 乌头碱 紫外-可见分光光度法 含量测定 |
Ultraviolet -visible Spectrophotometry Determine Content of Aconitine in Aconitum Excelsum Rrichb |
| |
| REN Wen-shuan,MA Ja,CHANG Fu-hou.Ultraviolet -visible Spectrophotometry Determine Content of Aconitine in Aconitum Excelsum Rrichb[J].Inner Mongolia Medical Journal,2010,42(2):136-139. |
| |
| Authors: | REN Wen-shuan MA Ja CHANG Fu-hou |
| |
| Institution: | 1.Department of Medicine/a>;Inner Mongolia Hospital/a>;Huhhot 010017 China/a>;2.Department of Pharmacology/a>;Inner Mongolia Medical College/a>;Huhhot 010059 China |
| |
| Abstract: | Objective：To determine the content of aconitine in Aconitum excelsum Rrichb.Methods：Ultraviolet-visible spectrophotometry was to used to measure absorbance in the wavelength of 520nm and to compute the weight of the ester-type alkaloids.Results：Concentration of aconitine within 15.1～75.5 μg/ml appears the favorable linear relationship.The regression equation was Y = 0.0086X＋0.13 r = 0.9993,this product contains ester-type alkaloids per gram may not be over 5 mg.Conclusion：The ultraviolet-visible spectrophotometry was the simple and accurate method to determine the content of aconitine in Aconitum excelsum Rrichb. |
| |
| Keywords: | Aconitum excelsum rrichb Aconitine Ultravioletvisible spectrophotometry Determination of content |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
