金雀异黄素对正常的和过氧化氢损伤的内皮细胞的影响(英文)

目的 :观察金雀异黄素 (genistein)对体外培养的血管内皮细胞 (ECV 3 0 4)生长的影响和对过氧化氢引起ECV 3 0 4细胞损伤的保护作用。方法 :将ECV 3 0 4细胞与金雀异黄素 0 .1～ 1 0 0 μmol·L-1共同孵育 2 4～ 96h ,通过MTT法测定细胞存活率。为了观察金雀异黄素对过氧化氢引起ECV 3 0 4细胞损伤的保护作用 ,将细胞分成 3组 ,分别为对照组、过氧化氢损伤模型组和金雀异黄素治疗组 ,治疗组ECV 3 0 4细胞先与金雀异黄素 6.2 5～ 1 0 0 μmol·L-1共同孵育 3 0min ,然后模型组和治疗组ECV3 0 4细胞均加入过氧化氢 1 0mmol·L-1孵育 ,3 0min后以MTT法测定OD值 ,以此反映各组ECV 3 0 4的生存情况。结果 :金雀异黄素对于正常ECV 3 0 4细胞生长的作用与浓度和作用时间有关 ,浓度为1 0 0 μmol·L-1时 ,作用 2 4,48h能明显促进ECV3 0 4生长 (P <0 .0 1 ) ,而作用 96h则表现为明显的抑制作用 (P <0 .0 5 ) ;浓度为 1 0 μmol·L-1时 ,作用48,72h对ECV 3 0 4细胞生长也有促进作用 (P <0 .0 5 ) ;而当金雀异黄素浓度降低为 0 .1 ,1 μmol·L-1时 ,对ECV 3 0 4细胞生长无明显影响。过氧化氢 1 0mmol·L-1可使ECV 3 0 4的存活率明显下降(P <0 .0 5 ) ;预先给予金雀异黄素 2 5 ,5 0 ,1 0 0 μmol·L-1能浓度依赖性地

Effect of genistein on proliferation of normal and hydrogen peroxide injured endothelial cells

AIM: To investigate the effect of genistein on the survival of normal human vascular endothelial cells (ECV 304) and the protection of genistein on injured ECV 304 induced by hydrogen peroxide(H2O2). METHODS: Normal ECV 304 was incubated with genistein 0.1-100 μmol*L-1 for 24-96 h, the survival rate of ECV 304 was then determined by tetrazolium assay (MTT). To evaluate the protective effect of genistein on ECV 304 from H2O2 injury, ECV 304 was divided into control, model, and genistein treatment groups. In genistein treatment group, ECV 304 was incubated with genistein 6.25-100 μmol*L-1 for 30 min. Then, H2O2 10 mmol*L-1 (final concentration) was added to ECV 304 in the model group and the genistein treatment group. Thirty minutes later, the survival conditions of ECV 304 were expressed by the OD values assessed by MTT assay. RESULTS: The effect of genistein on the survival rate of normal ECV 304 was related to both the concentration and the duration of drug exposure. Genistein 100 μmol*L-1 could significantly increase the survival rate of ECV 304 at 24, 48 h, but significantly decrease the survival rate at 96 h. Genistein 10 μmol*L-1 could slightly increase the survival rate of ECV 304 at 48, 72 h. There were no significant changes in the survival rate of the ECV 304 incubated with genistein 0.1, 1 μmol*L-1 at 24, 48, 72, 96 h. The viability of ECV 304 was significantly inhibited after the exposure to H2O2 10 mmol*L-1. This inhibition in the viability of ECV 304 was concentration-dependently reversed by the pretreatment with genistein 25, 50, 100 μmol*L-1, as compared with the model group. CONCLUSION: Genistein has dual effects on the survival rate of normal ECV 304 in vitro, which is related to the concentration and the duration of drug exposure. Genistein 25-100 μmol*L-1 can concentration-dependently protect ECV 304 against H2O2 injury.