981208冲剂抑制二乙基亚硝胺诱导的大鼠肝癌细胞增殖活性

目的：探讨中药981208冲剂对二乙基亚硝胺（DEN）诱癌过程中肝细胞增殖活性的影响。方法：建立DEN诱肝癌大鼠模型，采用免疫组化染色方法及计算机图像分析技术检测了981208冲剂对诱癌过程中肝细胞阳性表达细胞增殖核抗原（PCNA）、H－ras蛋白及诱癌后期溴化脱氧尿嘧啶（Brd-U)细胞标记指数的影响。结果：DEN诱肝癌过程中，PCNA、H-ras阳性表达呈逐渐增加趋势，两表达显正相关。981208冲剂可明显抑制诱癌过程中两蛋白的阳性表达，显降低诱癌后期Brd-U细胞标记指数。结论：中药981208冲剂可抑制大鼠肝癌形成过程中肝细胞增殖活性，从而延缓肝细胞癌变的过程。

Inhibitive effect of 981208 agent on cell proliferating of hepatocarcinogenesis induced by diethyl nitros-amine in rats

Objective: To investigate the influence of 981208 agent on cell proliferaton of hepatocarcinoma induced by diethyl nitrosamine(DEN). Methods: Rat hepatocarcinogenesis model induced by DEN was established and 981208 agent was used during the stage. Influence of 981208 agent on hepatocytes positive expression of proliferating cell nuclear antigen (PCNA), H ras and bromodeoxyuridine labeling index (Brd U LI) during DEN induced rat hepatocarcinogenesis was examined by immunohistochemical method and analyzed by computerized image technique. Results: Positive expression of H ras, PCNA increased gradually and worked synergically. Overexpression of 2 proteins was inhibited significantly during DEN induced hepatcacinogenesis and Brd U LI was reduced strikingly in later stage of model by 981208 agent. Conclusion: 981208 agent can inhibit proliferating character of hepatocytes and slow down the process of hepatocarcinogenesis induced by DEN in rats.