病理性瘢痕中结缔组织生长因子的表达及其意义

目的:观测结缔组织生长因子(Connective Tissue Growth Factor,CTGF)在瘢痕组织中的表达,以探讨其与病理性瘢痕形成的关系。方法:分别取正常皮肤20例、成熟瘢痕20例、增生性瘢痕20例和瘢痕疙瘩20例标本,经HE染色、原位杂交(SABC法)染色,用图象分析系统定量分析CTGF在不同组织中表达的差异性。结果:CTGF在增生性瘢痕、瘢痕疙瘩、瘢痕疙瘩边缘正常皮肤中呈强阳性表达,明显高于其在正常皮肤中的表达,差异具有统计学意义(P<0.05);其在成熟瘢痕、增生瘢痕边缘正常皮肤呈弱阳性表达,与正常皮肤相比差异无统计学意义(P>0.05)。结论:CTGF在病理性瘢痕的发生发展中起重要作用。

The expression and significance of CTGF in pathologic scars

Objective To observe the expression of CTGF in pathologic scars, and to investigate the relationship between which and pathologic scars. Methods The expression of CTGF was examined through HE staining and in situ hybridization histochemistry (ISHH) (20 normal skins, 20 mature scars, 20 hyperplastic scars,20 keloidscars). Then the distribution was analysised quantitatively using image analytical system. Results Compared with common skins, the expression of CTGF in hyperplastic scars, keloid and adjacent uninvolved skins of keloid was significantly increased (P<0.05). Which increased indefinitely in mature scars and adjacent uninvolved skins of hyperplastic scars, and there was no statistically significance compared with common skins (P<0.05). Conclusion CTGF may play important roles in the occurrence and development of pathologic scars.