妊娠相关血清蛋白-A对内皮细胞生理功能的影响

目的：观察妊娠相关血清蛋白-A（PAPP-A）在体外对内皮细胞生理功能的影响。方法：体外培养人脐静脉内皮细胞株(HUVEC)，随机分为4组。分别给予不同浓度(0，50,100及200ng/mL)的PAPP-A刺激，于0,12,24和48 h收集上清液和细胞，用硝酸还原酶法测定一氧化氮（NO）的浓度，用免疫组织化学法测定内皮素-1（ET-1）的表达。结果：在不同的时间点，随着PAPP-A浓度的增加，上清液NO的浓度逐渐降低，与对照组相比差异具有统计学意义(P＜0.05)。随着PAPP-A浓度的增加，ET-1的表达呈浓度依赖性增加，与对照组相比差异具有统计学意义(P＜0.05)。结论：PAPP-A可能通过减少内皮细胞NO的分泌，上调ET-1的表达来影响内皮细胞的功能。

Effect of pregnancy-associated plasma protein-A on the function of endothelial cells

ObjectiveTo determine the effect of pregnancy-associated plasma protein A (PAPP-A) on the function of vascular endothelial cells (VEC). MethodsHuman umbilical vein endothelial cell (HUVEC) line, derived from human umbilical vein, was cultured in vitro with PAPP-A at 0, 50, 100, and 200 ng/mL for 0, 12, 24, and 48 hours, respectively. Nitric oxide (NO) levels and endothlin-1 (ET-1) levels were determined by spectrophotometer and immunehistory. ResultsThe NO levels of HUVECs in the PAPP-A groups were significantly lower than those in the control group (P＜0.05). The ET-1 levels of HUVECs in the PAPP-A groups were significantly higher than those in the control group (P＜0.05). The changes were all dose-dependent. ConclusionPAPP-A may affect the function of vascular endothelial cells by reducing the secretion of NO and increasing the level of ET-1.