BRF2在慢性鼻-鼻窦炎伴鼻息肉发病过程中的作用研究

目的 研究BRF2在慢性鼻-鼻窦炎伴鼻息肉中的表达及意义。 方法 收集山东大学第二医院耳鼻咽喉头颈外科2016至2017年慢性鼻-鼻窦炎伴鼻息肉(CRSwNP)手术标本37例及单纯鼻中隔偏曲手术标本18例,采用免疫荧光染色检测BRF2在慢性鼻-鼻窦炎伴鼻息肉组织中的表达及定位;脂多糖(LPS)刺激体外培养人鼻黏膜上皮细胞后,采用免疫荧光检测BRF2在细胞中的表达及定位,Western bloting及实时荧光定量PCR检测BRF2和内质网应激相关蛋白GRP78在细胞中的表达。 结果 BRF2在慢性鼻-鼻窦炎伴鼻息肉组织中表达量上调并在胞浆中的分布增多;在体外,LPS刺激可诱导鼻黏膜上皮细胞中BRF2表达上调并在胞浆中的分布增多,并发现内质网应激相关蛋白GRP78表达上升。 结论 BRF2可能介导了内质网应激,从而参与了慢性鼻-鼻窦炎伴鼻息肉的发病过程。

BRF2 in the pathogenesis of chronic rhinosinusitis with nasal polyps

Objective To study the expression and significance of BRF2 in chronic rhinosinusitis with nasal polyps. Methods 37 surgical specimens of chronic rhinosinusitis with nasal polyps(CRSwNP)and 18 surgical specimens of simple deviation of nasal septum were collected from 2016 to 2017 in the Department of Otorhinolaryngology Head and Neck Surgery, Second Hospital of Shandong University. Immunofluorescence staining was used to detect the expression and localization of BRF2 in chronic rhinosinusitis with nasal polyps. After cultured human nasal mucosa epithelial cells were stimulated with Llipopolysaccharides(LPS)in vitro, the expression and localization of BRF2 in cells were detected by immunofluorescence staining. Western blotting and quantitative real-time PCR were used to detect the expression of BRF2 and the endoplasmic reticulum stress-related protein GRP78 in cells. Results The expression of BRF2 in chronic rhinosinusitis with nasal polyps was up-regulated in whole cells and was particularly increased in the cytoplasm fraction. In vitro, LPS stimulation can induce the up-regulation of expression of BRF2 to be up-regulated in nasal mucosal epithelial cells and increase the distribution of BRF2 in the cytoplasm, and tThe expression of endoplasmic reticulum stress-related protein GRP78 was also increased. Conclusion BRF2 may mediate endoplasmic reticulum stress, which is involved in the pathogenesis of chronic rhinosinusitis with nasal polyps.