IL-31、IL-33及其受体ST2在难治性鼻-鼻窦炎中的表达及意义

目的 研究IL-31、IL-33及其受体ST2在难治性鼻-鼻窦炎(RRS)患者鼻黏膜组织中的表达,初步探讨其在RRS发病机制中的作用及意义。 方法 研究设立难治组、初治组及对照组。采用HE染色方法观察组织形态和检测嗜酸性粒细胞数(EOS);采用免疫组织化学法检测组织中IL-31、IL-33及ST2蛋白的相对含量;采用实时荧光定量PCR技术在mRNA水平检测IL-31、IL-33及ST2蛋白的表达;外周血检测血清总IgE的表达水平。 结果 (1) 难治组、初治组的嗜酸性粒细胞数均高于对照组,难治组高于初治组;(2)免疫组织化学染色中,IL-31、IL-33在难治组、初治组及对照组均有表达,难治组、初治组表达量高于对照组,难治组高于初治组。ST2表达量难治组、初治组均高于对照组,但难治组与初治组之间差异无统计学意义;(3) 在mRNA表达水平上, IL-31、IL-33在难治组及初治组中的表达较对照组上调,难治组高于初治组;ST2表达量难治组、初治组均高于对照组,但难治组与初治组之间差异无统计学意义;(4)外周血总IgE值三组间差异无统计学意义。 结论 IL-31、IL-33及其受体ST2可能在慢性鼻-鼻窦炎患者,尤其在难治性鼻-鼻窦炎患者发病机制中发挥重要作用;嗜酸性粒细胞浸润可能在难治性鼻-鼻窦炎的发展中有重要作用。

The expression and significance of interleukin-31, and interleukin-33 and its receptor ST2, in refractory rhinosinusitis

Objective To study the expression of interleukinIL-31(IL-31), and interleukin-33(IL-33)and its receptor ST2, in the nasal mucosa of patients with refractory rhinosinusitis(RRS), and to investigate their role in the pathogenesis of RRS. Methods In this study, a refractory group, an initial treatment group, and a control group were established. The hematoxylin and eosin staining method was used to observe the morphology and detect the number of eosinophils(EOS)present in the mucosal tissues. The relative levels of IL-31, IL-33, and ST2 in the tissues were detected by immunohistological examination.Quantitative fluorescence real-time polymerase chain reaction was used to detect the mRNA levels of IL-31, IL-33, and ST2, and total serum immunoglobulin E(IgE)expression in the peripheral blood. Results (1)The number of EOS in the refractory group and initial treatment group were higher than that in the control group. Further, the number of EOS in the refractory group were higher than that in the initial group. (2) On immunohistological examination, the expression levels of IL-31 and IL-33 proteins in the refractory group and the initial treatment group were higher than those in the control group. Additionally, the expression levels of both IL-31 and IL-33were higher in the refractory group than in the initial treatment group. The expression of ST2 in the refractory group and the initial treatment group was higher than that in the control group, but there was no significant difference in the expression of ST2 between the refractory group and the initial treatment group. (3) At the level of mRNA expression, the levels of IL-31 and IL-33 in the refractory group and the initial treatment group were higher than those in the control group. Furthermore, the levels of IL-31 and IL-33 mRNA in the refractory group were higher than those in the initial treatment group. The ST2 mRNA levels in the refractory group and the initial treatment group were higher than that in the control group, but there was no significant difference between the refractory group and the initial treatmentgroup. (4) There was no significant difference between the three groups in the total IgE levels in the peripheral blood. Conclusion IL-31, and IL-33 along with its receptor ST2, may play an important role in the pathogenesis of chronic rhinosinusitis, especially in patients with RRS. Eosinophilic infiltration may also play an important role in the development of RRS.