非浓缩尿蛋白电泳的临床应用

目的 尿液中各种蛋白质进行分离，用于区分尿蛋白类型。方法 应用十二烷基磺酸钠-琼脂糖凝胶进行非浓缩尿蛋白电泳，对86例肾脏疾病患者尿液样本（其中16例经病理活检确诊）进行分析。结果 根据尿蛋白电泳剖象可以区分为肾小球性、肾小管性、混合性、溢出性及生理性蛋白尿。16例经肾活检患者尿标本，其中肾小球蛋白尿12例，混合性蛋白尿3例，1例生理性蛋白尿。结论 本法具检测敏感性好，诊断符合率高，方法简便，省时，经扫描后可获半定量结果，胶片易于保存等优点。

Clinical application of protein electrophoresis using non-concentrated urine samples

Objectives To analyze the various protein components in urine and to define the types of proteinuria. Methods Sodium dodecyl sulfate argarose gel electrophoresis was used to analyze 86 non concentrated urine samples from patients with various renal injury. Results According to the images of urine protein electrophoresis, proteinuria can be classified into five types: glomerular, tubular, mixed, over flow, and physiological. Results from 16 cases of renal biopsy showed glomerular type, in 12 cases mixed type in 3, physiological type in 1. Conclusion This method is highly sensitive, simple, less time consuming and easy for long term preservation of results.