骨髓间充质干细胞移植对嘌呤霉素氨基核苷肾病大鼠足细胞修复作用的研究

目的：探讨骨髓间充质干细胞(bone mesenchymal stem cells,BMSCs)移植对嘌呤霉素氨基核苷(puromycin aminonucleoside,PAN)肾病大鼠足细胞修复作用及其对裂孔隔膜分子Nephrin表达的影响，为BMSCs移植治疗肾病综合征提供实验依据。方法：45只雄性Sprague-Dawley大鼠随机分为 3组，每组15只。模型组：一次性腹腔注射PAN（0.15 mg/g）造模；BMSCs组：PAN造模+BMSCs移植（细胞移植前经Brdu标记示踪）；对照组：不予以造模。实验第10天杀鼠，留取标本行血、尿、肾组织超微结构检测，免疫组化检测肾脏Brdu标记阳性细胞的表达，半定量RT-PCR及Western blot方法检测Nephrin 表达变化。结果：模型组大鼠出现大量蛋白尿、血白蛋白降低、胆固醇升高,伴有广泛足突的融合。与模型组比较，BMSCs组尿蛋白、血胆固醇降低,血白蛋白回升及足突融合现象改善，差异有统计学意义（P＜0.05）；免疫组化显示，BMSCs组肾组织中可见阳性细胞，模型组及对照组未见阳性细胞；半定量RT-PCR及Western blot显示，模型组Nephrin相对表达量较对照组明显下降，BMSCS组与模型组比较有所增加，差异有统计学意义（P＜0.05)。结论：BMSCs移植对PAN肾病大鼠足突融合有一定的改善，Nephrin表达的变化可能在其中发挥了作用。［中国当代儿科杂志，2010，12（6）：483-487］

Bone mesenchymal stem cell transplantation repairs glomerular podocytes in rats with puromycin aminonucleoside-induced nephrosis

Objective To investigate the effect of bone mesenchymal stem cell(BMSC)transplantation on repair of glomerular podocytes and on the Nephrin expression in rats with puromycin aminonucleoside(PAN)-induced nephrosis.Methods Forty-five Sprague-Dawley rats were randomly divided into three groups(n=15 each):a nephrosis model group that received a single intraperitoneal injection of PAN(0.15 mg/g);a BMSC transplantation group that received a single intraperitoneal injection of PAN(0.15 mg/g)followed by BMSC transf...