Adenovirus-mediated PTEN gene transfection suppresses growth and promotes chemosensitivity of endometrial carcinoma |
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Authors: | Liu Yuhuan Wang Jiaqi Yang Peili Hu Jingjing Chen Chao Ji Mei Hui Ning Yu Chaoqin Cai Zailong |
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Institution: | [1]Department of Gynecology and Obstetrics, Changhai Hospital, Second Military Medical University, Shanghai 200433, China [2]Clinical Research Center, Changhai Hospital, Second Military Medical University, Shanghai 200433, China [3]Department of Gynecology and Obstetrics, Hospital of Beijing Municipal Corps of Chinese People & Armed Police Force, Beijing 100027, China [4]Department of Chinese Traditional Medicine, Changhai Hospital, Second Military Medical University, Shanghai 200433, China |
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Abstract: | ObjectiveTo determine the potential of sustained transgene expression by intratumoral injection of Ad-PTEN in the nude mouse model of endometrial carcinoma.Methods and ResultsWe constructed recombinant adenovirus carrying the wild-type PTEN gene (Ad-PTEN). RL95-2 cells, an endometrial carcinoma cell line lacking PTEN function, was infected with Ad-PTEN and showed increased expression of PTEN and chemosensitivity to doxorubicin, decreased proliferation rate, and elevated apoptosis and G0/G1 arrest. Furthermore, the tumorigenicity of these cells was also completely suppressed. These results indicated that gene therapy with Ad-PTEN could significantly inhibit the endometrial carcinoma xenografts growth in nude mice by intratumoral injection, induce apoptosis of tumor cells, and reduce expression of proliferating cell nuclear antigen (PCNA). Immunohistochemistry analysis also showed that the expression of progesterone receptors (PR) in Ad-PTEN treated tumor cells were induced, while P-glycoproteins (P-gp) and estrogen receptors (ER) decreased significantly.ConclusionPTEN may play an important role in the development of endometrial carcinoma. Our findings cast new lights for treatment of endometrial carcinoma. |
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Keywords: | Endometrial carcinoma Gene therapy Chemosensitivity Apoptosis |
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