大鼠创伤性脑损伤模型的建立

目的：建立一种控制性皮层撞击脑损伤大鼠模型。方法：采用BenchmarkTM颅脑损伤撞击器制备大鼠创伤性脑损伤,并在伤后1、3、5、7、28 d借助Morris水迷宫评价其认知功能,同时观察损伤部位及其邻近区域的形态学改变。结果：脑损伤大鼠寻找隐藏平台所用时间显著高于假手术组。损伤部位及海马回可见神经细胞肿胀、坏死,胶质细胞明显增生。经铜银染色可见神经元及其轴突溃变。结论：建立的大鼠控制性皮层撞击脑损伤模型重复性好,适合脑保护药物的药效学筛选。

Establishment of traumatic brain injury model in rats

AIM：To establish a rat model for controlled cortical impact brain injury. METHODS： Traumatic brain injury（TBI） model in rats was duplicated by controlled cortical impact brain injury method with a BenchmarkTM stereotaxic impactor.Cognitive function was evaluated at 1,3,5,7,28 day after TBI with Morris water maze.And then morphological changes was carefully inspected in the injured part and adjacent area.RESULTS：Compared with the sham-operated rats,the time for brain injured rats to find hidden platform were significantly prolonged.Neuronal cells were swollen or loss,and glia cells remarkably increased in the injured sites and hippocampus areas.Neuron and its axon degeneration was observed by using curpric-silver straining technique.CONCLUSION：The rat model for controlled cortical impact brain injury is reproducible and suitable for the pharmacodynamic screening of brain protective drugs.