大蒜素对二甲基亚硝胺诱发的肝纤维化大鼠的保护作用

目的　研究大蒜素对实验性肝纤维化大鼠的保护作用。方法　 SD大鼠随机分为 5组 :正常对照组、模型组(给等量蒸馏水 )、大蒜素 (11、2 2 mg/ kg)组、秋水仙碱 (0 .15 g/ kg)组。二甲基亚硝胺 (DMN)诱发大鼠肝纤维化模型 ,各组在造模开始时 ig给药 ,实验共进行 4 2 d。腹主动脉取血 ,制备血清 ,进行肝功能、血脂、肝细胞病理学检查。结果　大蒜素各组均能明显降低实验性肝纤维化大鼠血清丙氨酸转氨酶 (AL T)、谷草转氨酶 (AST) (P<0 .0 5、0 .0 1)水平 ,以大蒜素 2 2 mg/ kg组作用明显 ,而秋水仙碱对肝功能没有明显的改善作用 (P>0 .0 5 )。大蒜素均能明显降低血清甘油三酯 (TG)、胆固醇 (TC)水平 ,提高血清白蛋白 (AL B)含量。病理学检查结果 ,大蒜素组肝细胞的坏死、空泡变性、出血及脂肪沉积较模型组和秋水仙碱组明显减轻 ,炎症细胞明显减少。电镜检查结果显示 ,大蒜素 11mg/ kg组肝细胞坏死、自溶较模型组明显减轻 ,大蒜素 2 2 mg/ kg组肝细胞接近正常。结论　大蒜素对 DMN所致肝损伤具有保护作用。

Protective effect of garlicin on hepa ticf ibrosis induced by dimethyln itrosa mine inrats

Object To investigate the effect of garlicin on dimethylnitrosamine (DMN)-induced hepatic fibrosis in rats. Methods SD rats were randomly divided into five groups: normal group, model group, garlicin (11, 22 mg/kg) groups, and colchincine (0.15 g/kg) group. Except for normal group, rats in other four groups were given DMN by ip to induce hepatic fibrosis. Rats in all groups were given corresponding drugs by ig, and the treatment was consecutively carried out for 42 d. After the last time of treatment, the celiac arterial blood of all rats were taken. The serum liver function test, albumin, TG, and TC, pathobiological examination of hepatic cells were analyzed. Results In the groups treated with garlicin, the serum levels of ALT and AST were reduced. Albumin was increased significantly (P<0.01, 0.05). Garlicin 22 mg/kg group has the most significant effect on ALT and AST. But the colchincine did not show any beneficial effect on liver functions (P>0.05). TG and TC were decreased in garlicin groups. The pathobiological examination showed that the necrosis, vacular degeneration, hemorrhagic, and accumulation of TG in the liver of garlicin groups were less severe than that in the model group and colchincine group. Electronic microscope examination showed that in garlicin 11 mg/kg group the liver necrosis and isophagy were less severe than that in the model group. The hepatic cells in garlicin 22 mg/kg group were as normal as that in negative control group. Conclusion It is suggested that garlicin have protective effect on hepatic fibrosis induced by DMN in rats.