Differential requirements for Ca2+ concentrations for catecholamine release and biosynthesis in isolated bovine adrenal chromaffin cells |
| |
Authors: | Hitoshi Houchi Masanori Yoshizumi Yasuko Ishimura Motoo Oka |
| |
Institution: | (1) Department of Pharmacology, School of Medicine, The University of Tokushima, 3-18-15Kuramoto, 770 Tokushima, Japan |
| |
Abstract: | Differential effects of Ca2+] on catecholamine release and biosynthesis in isolated bovine adrenal chromaffin cells were investigated. Carbamylcholine, an agonist of the nicotinic and muscarinic acetylcholine receptor, or Na+ deprivation in the incubation medium, stimulated catecholamine release and biosynthesis in these cells. The concentrations of extracellular Ca2+] which stimulate catecholamine biosynthesis were less than those which stimulate catecholamine release. An increase in intracellular levels of free Ca2+ (Ca2+]i) induced by Na+ deprivation was dependent on extracellular Ca2+]. These results indicate that, in bovine adrenal chromafn cells, catecholamine biosynthesis is regulated by lower levels of Ca2+]i than is catecholamine release. |
| |
Keywords: | Biosynthesis Release Catecholamine Calcium ion Chromaffin cell |
本文献已被 SpringerLink 等数据库收录! |
|