骨髓单核细胞移植对脊髓横断大鼠神经营养因子-3和突触素表达的影响

目的:探讨骨髓单核细胞(BMMNCs)移植对脊髓横断(SCT)大鼠脊髓组织中神经营养因子-3(NT-3)和突触素(SYN)表达的影响。方法:146只Winstar大鼠(SPF级,雌雄不限)随机分为3组:SCT组49只,建立SCT模型;实验组49只,建立SCT模型,给予BMMNCs移植治疗;假手术组48只,切除对应的椎板不损伤脊髓;各组大鼠分别于术后5、7、14和21d行后肢功能BBB评分,RT-PCR和免疫组织化学技术检测受损脊髓组织中NT-3和SYN的表达。结果:术后5、7、14和21d各时间点各组大鼠后肢BBB评分比较,SCT组和实验组均低于假手术组(P0.05),且SCT组更低于实验组(P0.05)。术后5、7、14和21d各时间点NT-3阳性细胞数及NT-3mRNA比较,SCT组和实验组均高于假手术组(P0.05),且SCT组更低于实验组(P0.05)。SYN阳性颗粒数及SYN mRNA比较,SCT组和实验组均低于假手术组(P0.05),且SCT组更低于实验组(P0.05)。结论:BMMNCs能够上调局部损伤脊髓NT-3和SYN的表达。

Effects of bone marrow mononuclear cells transplantation on the expression of NT-3 and SYN in rats with spinal cord injury

Objective：To explore the effects of bone marrow mononuclear cells （BMMNCs） on the expression of neurotro-phin-3 （NT-3） and synaptophysin （SYN） in the injured spinal cord of rats with complete transection of the spinal cord （SCT） .Methods：The rat model of complete SCT was set up ,and 146 rats were divided randomly into Sham group （n=48） ,SCT model group （n=49） and experimental group （n=49） .Sham group ,removing the corresponding lamin and sutu-ring muscle and skin separately ;SCT model group ,establishing SCT models ;experimental group ,given BMMNCs transplan-tation in the SCT models .On the postoperative day （POD） 5 ,7 ,14 and 21 ,the rat hindlimb was scored by the scale of Bas-so ,Beattie and Bresnahan （BBB Grading） ,and the expression levels of NT-3 and SYN were detected by the immunohisto-chemistry and RT-PCR .Results：On the POD 5 ,7 ,14 and 21 ,BBB scores in experimental group and SCT model group were significantly lower than in Sham group （P〈0 .05） ,and those in SCT model group were lower than in experimental group （P〈0 .05） .RT-PCR and immunohistochemistry revealed the expression levels of NT-3 mRNA and number of NT-3 posi-tive cells in experimental group and SCT model group were significantly increased as compared with Sham group （ P〈0 .05） ,and those in SCT group were higher than in experimental group （P〈0 .05） .The positive particles of SYN and SYN mRNA in experimental group and SCT model group were significantly increased as compared with Sham group （P〈0 .05） , and those in SCT model group were increased as compared with those in experimental group （P〈 0 .05） .Conclusions ：BMMNCs can promote the expression of NT-3 and SYN in injured spinal cord tissues ,which may be one of the mechanisms by which BMMNCs repair spinal cord injury .