舌癌组织中淋巴管密度与淋巴道转移关系的研究

目的：研究舌癌组织中淋巴管密度（lymphatic vessel density,LVD）和舌鳞状细胞癌患者淋巴道转移的相关性。方法：舌癌样本来自138例实施舌癌切除术的患者。利用单克隆抗体D2-40进行免疫组织化学染色。对照正常组织和肿瘤组织中LVD的差异。利用Spearman相关分析评估舌癌患者的LVD和临床病理因素的联系。结果：舌癌组织中LVD明显高于正常组织（P〈0.01）。已发生转移的肿瘤组织中LVD明显高于未转移肿瘤组织（P〈0.01）。LVD与舌癌患者T分期、分级、淋巴结状态和临床分期（r=0.229,0.221,0.794,0.740）具有紧密联系。结论：LVD有规律的增长可能是舌癌发展的一个重要特征,有助于预测舌癌患者早期的淋巴转移。

Investigating the relations between the lymphatic vessel density in OTSCC tissues and lymphatic metastasis

Objective：To investigate the relationship of the lymphatic vessel density（LVD） in oral tongue squamous cell carcinomas（OTSCC）tissues and lymphatic metastasis of OTSCC patients.Methods： OTSCC samples were obtained from 138 patients undergoing tumor resection.Immunohistochemical staining was performed using monoclonal antibodies D2-40.LVD in normal and tumor tissue was compared.Additionally,the relations between LVD and clinicopathologic factors of OTSCC patients were evaluated by Spearman correlation analysis.Results： LVD in cancer tissues were significantly higher than those in normal tissue（P0.01）.Similar results were obtained from the comparison between metastatic tumor and non-metastatic tumor（P0.01）.LVD was closely related with T stage,grade,lymph node status and clinical stage（r=0.229,0.221,0.794,0.740）.Conclusions： Up-regulation of LVD might be an important feature of OTSCC progression,which may exert their function and favorably predicted lymphatic dissemination for relative early stage of OTSCC patients.