高效液相色谱法测定兔血浆中决明子苷B浓度

目的:建立兔血浆中决明子苷B的高效液相色谱测定方法。方法:血浆样品经甲醇沉淀蛋白,上清液直接进样分析。采用μ-Bondapak C_(18)柱,以乙腈-水-四氢呋喃-冰醋酸(20:76.5:3.0:0.5)为流动相,流速为1ml/min,检测波长为278nm。结果:血浆标准曲线C=9.102×10~(-2)+4.871×10~(-5)R,r=0.9 999。回收率为(100.8±1.139)％,精密度能满足分析的要求,血浆中药物最低检测浓度为0.05μg/ml。结论:本方法灵敏、准确,适用于决明子苷B药动学研究。

Determination of Cassiaside B in Rabbit Plasma by HPLC

OBJECTIVE: To establish a HPLC assay for determining cassiaside B in rabbit plasma .METHODS: The proteinin sample was first denatured with methanol .The μ-- Bondapak C_(18) colurnn(3.9mm×300mm, 10μm) was used with a mobilephase of acetonitrile -- water -- THF -- glacial acetic acid(20:76.5:3.0:0.5) .The flow rate was 1.0ml/ min .Detecting wave-length was 278nm .RESULTS: The caliblation curve of cassiaside B was C=9.102×10~(-2)+4.871×10~(-5)R(r=0.9999) .Theaverage recovery of cassiaside B in plasma was (100.8±1.139)% .The relative standard deviations of intra -- day and inter -- dayassay were less than 2.08% .The detection limit in plasma was 0.05μg/ml .CONCLUSION: The method is sensitive and accu-rate. It is suitable for the pharmacokinetic study of cassiaside B.