荧光定量聚合酶链反应检测儿童病毒性脑炎病原体的研究

目的 探讨荧光定量聚合酶链反应(FQ PCR)检测儿童病毒性脑炎脑脊液(CSF)中的病原体的价值。方法 应用定性聚合酶链反应(PCR)对2002年1月至2004年10月保定市儿童医院收治的病毒性脑炎患儿78例的CSF标本筛选,检测最常见的引起病毒性脑炎的6种病毒,对单纯疱疹病毒(HSV)，柯萨奇病毒(CV)，埃可病毒(EV)阳性者做FQ-PCR检测，其中HSV分别在3个时点(治疗前、治疗中、出院时)检测DNA拷贝数。结果 定性聚合酶链反应阳性标本FQ PC检测100%阳性,HSV-DNA拷贝数在3个时点有统计学差异，病毒核酸拷贝数与临床表现一致。结论 FQ-PCR可作为儿童病毒性脑炎早期诊断的有效方法之一，动态观察HSV-DNA含量有助于指导治疗，估计疾病的发展和预后. Abstract Objective To discuss the value of using FQ-PCR to check pathogen in children viral encephalitis.Methods From Janurary,2002 to October,2004,PCR was used to select the children of viral encephalitis and check the six kinds of common virus which cause viral encephalitis and then we used FQ-PCR to check the children with HSV(+),CV(+)EV(+) while checking the copied number of HSVE-DNA before,during and after treatment.Results It was 100% that FQ-PCR checked the sample of PCR (+).The number of HSV DNA was different at three time points. The copied number of virus DNA was consistent with clinical symptom.Conclusion FQ-PCR is one of the effective methods to diagnose the children's viral encephalitis.Observing the number of HSV DNA is beneficial for instructing,treating and estimating the development and future disease. Key words Children’viral encephalitis;Pathogen;FQ-PCR;PCR

The research of fluorescence quantitative polymerase chain reaction (FQ-PCR) method to test children virus encephalitis.

Abstract Objective To discuss the value of using FQ-PCR to check pathogen in children viral encephalitis.Methods From Janurary,2002 to October,2004,PCR was used to select the children of viral encephalitis and check the six kinds of common virus which cause viral encephalitis and then we used FQ-PCR to check the children with HSV(+),CV(+)EV(+) while checking the copied number of HSVE-DNA before,during and after treatment.Results It was 100% that FQ-PCR checked the sample of PCR (+).The number of HSV DNA was different at three time points. The copied number of virus DNA was consistent with clinical symptom.Conclusion FQ-PCR is one of the effective methods to diagnose the children's viral encephalitis.Observing the number of HSV DNA is beneficial for instructing,treating and estimating the development and future disease.