荧光定量聚合酶链反应在临床检测沙眼衣原体和解脲支原体的应用

目的:检测不孕妇女和自然流产患者生殖道沙眼衣原体（CT）和解脲支原体（UU）的感染率。方法:采用荧光定量聚合酶链反应方法,检测223例生殖门诊不孕妇女和自然流产患者宫颈分泌物CT DNA和UU DNA,同时选择50例正常早孕妇女宫颈分泌物作为对照。结果:原发不孕组妇女宫颈分泌物CT DNA和UU DNA阳性检出率分别为26.0％（平均拷贝数为:2.18X105/mL）和26.7％（平均拷贝数5.3 X105/mL）,高于正常早孕组（P<0.05）;继发性不孕组CT和UU阳性检出率分别为23.8％（平均拷贝数为:1.42X105/mL）和34.4％（平均拷贝数为2.77X105/mL）,与正常早孕组相比有显著差异（P<0.05）;习惯性流产组CT和UU阳性检出率分别为17.9％（平均拷贝数为:0.53X105/mL）和21.4％（平均拷贝数为:1.5 X105/mL）与正常早孕组相比无统计学意义。结论:荧光定量PCR可作为临床常规检测不孕妇女CT和UU感染的重要手段。

Detection of Chlamydia trachomatis(CT) and Ureaplasma Urealyticum(UU) Infection with Fluorescence Quantitative Polymerase Chain Reaction

ve: To undertake the genital infections rate of chlamydia trachomatis (CT) and ureaplasma urealyticum (UU) in infertile and natural abortive women. Methods: FQ-PCR technique was used to in-vestigate CT and UU infection from the cervical secretion of 223 cases of infertile women and 50 cases of normal early intrauterine pregnancy women as controls. Results: Significant differences (P<0. 05) were found between the CT and UU DNA positivity rates of the group of primary infertile women and normal controls (primary infertile women CT 26%; average copy number 2. 18X105/mL; UU 26. 7%; average copy number 2. 77X105/mL). Significant differences (P<0. 05) were also found between the CT and UU DNA positivity rates of the group of secondary infertile women and normal controls (secondary infertile women CT 23. 8%, average 1. 42X105/mL, UU 34. 4%, average copy number 2. 77X105/rnL); no signif-icant differences were found between the CT and UU DNA positivity rates of the group of habitual abor-tion women (CT 17. 9%, average copy number 0. 53X105/mL, UU 21. 4%, average copy number 1. 5X 105/mL) and controls. Conclusion: FQ-PCR can be used as a routine method for the diagnosis of CT and UU infection in infertile women.