KAI1基因对宫颈癌细胞增殖和侵袭能力的影响

目的 探讨肿瘤转移抑制基因KAI1对宫颈癌CaSki细胞体外增殖和侵袭能力的影响.方法 通过脂质体转染技术,将真核表达质粒pCMV-KAI1导入低表达的宫颈癌CaSki细胞,免疫组化及实时荧光定量RT-PCR法检测转染前后细胞内KAI1蛋白和mRNA的表达,通过四甲基偶氮唑蓝(MTT)比色法及Transwell侵袭力小室测定KAI1基因对细胞增殖能力及侵袭能力的影响.结果 转染目的基因的CaSki细胞中KAI1 mRNA水平及蛋白表达明显增强(P<0.05),细胞体外增殖能力明显弱于未转染组和转染空载体组(P<0.05),细胞穿膜数量明显少于未转染组和转染空载体组(P<0.05).结论 肿瘤转移抑制基因KAI1对宫颈癌细胞体外增殖及侵袭能力有一定抑制作用.

Effect of Metastasis Suppressor Gene KAI1 on the Proliferation and Invasive Ability of Cervical Carcinoma Cells

Objective To explore the effect of metastasis suppressor gene KAI1 on the proliferation and invasive ability of cervical cancer cell line CaSki. Methods pCMV-KAI1 cDNA plasmid was transferred into cervical carcinoma cell line CaSki by liposome, which had low level of endogenous KAI1 expression. The expressions of KAI1 protein and mRNA were determined by immunohistochemistry and real-time fluorescence quantitative PCR (RT-PCR), the proliferation of KAI1-transfected CaSki cells was investigated by MTT assay and the invasive ability of these cells was evaluated by in vitro invasion assays. Results After the transfection of pCMV-KAI1 cDNA, the level of KAI1 mRNA and protein expression in CaSki cell were increased (P<0.05), while the cell proliferation was suppresssed, and the migrative ability of passing through the membrane filte also decreased evidently (P<0.05). Conclusion The KAI1 metastasis suppressor gene suppressed the ability of proliferation and invasion of cervical cancer cell CaSki in vitro.