新城疫病毒对肿瘤细胞生物学行为的影响

目的：研究新城疫病毒（NDV）对肿瘤细胞的作用。方法：采用空斑试验观察NDV对鸡胚成纤维细胞（CEF）的作用。通过细胞抑制试验，琼脂糖凝胶电泳，TUNEL染色，细胞骨架染色及细胞唾液酸含量测定等，观察NDV对肿瘤细胞的影响。结果：NDV对CEF，HeLa及Hep-2等肿瘤细胞可产生明显的病变，而对人的羊膜细胞（Wish细胞）无明显影响；对Hela细胞和Hep-2细胞能产生明显的生长抑制作用。但无量效依赖关系。NDV还可导致以凋亡为主的细胞死亡。引起Hela细胞和Hep-2细胞的细胞骨架发生改变，以及去除肿瘤细胞表达唾液酸的作用。结论：NDV能选择性地作用于人肿瘤细胞。抑制肿瘤细胞的生长，导致肿瘤细胞发生凋亡，有望成为一种有效的抗肿瘤生物制剂。

The Effect of newcastle disease virus on the biological behavior of tumor cells

AIM: To investigate the anti-tumor effect of newcastle disease virus (NDV). METHODS: Plaque formation test was used to investigate the effect of NDV on chicken embryofi-broblasts( CEF). Cell suppression test, agarose gel electro-phoresis, cytoskeleton staining, fluorescence staining, TUNEL staining, and sialic acid content determination were used to observe the influence of NDV on several human tumor cells. RESULTS: The Plaque formation was observed in chicken embryo fibroblasts. NDV could lead to the apparent cytopathy of BHK, Hela and Hep-2 tumor cells, but has no apparent effect on Wish cells. The strong sup-pressive effect of NDV on the growth of these tumor cells was found without dose dependence. The optimal dose of NDV could induce the death of tumor cells which was mainly apoptosis, as showed by the classical DNA ladder in DNA gel electrophoresis. NDV also resulted in the changes of cytoskeleton and decreased the level of sialic acid contents on tumor cells. CONCLUSION: NDV may be a potential anti-tumor agent.