三七花提取液对小鼠的致突变作用研究

目的： 研究三七花提取液对小鼠的致突变作用。方法：分别以不同剂量(10 000、5 000、2 500 mg/kg)的三七花提取液经口灌胃染毒小鼠，每日1次，连续2 d，于第2次染毒6 h后处死小鼠，检测骨髓嗜多染红细胞微核率。另按上述剂量和分组经口灌胃连续染毒小鼠5 d，于首次染毒后第35天处死，观察精子畸形率。两项实验同时设阴性对照组(纯水)和阳性对照组(环磷酰胺，40 mg/kg)。结果：与阴性对照组比较，三七花提取液各剂量组均无致小鼠骨髓嗜多染红细胞微核作用和精子畸形作用。结论：在本实验条件下，三七花提取液对小鼠无致突变作用。

Study of the mutagenic action of notoginseng flower extract on mice

OBJECTIVE：To study the mutagenic action of notoginseng flower extract on mice. METHODS：The mice were treated by oral gavages twice 24 hours apart with notoginseng flower extract of different doses （10 000,5 000,2 500 mg/kg）. Animals were killed after 6 h of the last treatment,and then the micronucleus frequency （MNF） of polychromatic erythrocytes （PCE） in bone marrow were measured. In addition,at the above doses and groups another group of mice were treated by oral gavages once a day for 5 consecutive days. The mice were killed after 35 days of the initial treatment and the sperm deformity rate was observed. The two experiments also consisted of the negative control group （pure water） and positive control group （cyclophosphamide 40 mg/kg） at the same time. RESULTS：Every dosage group of notoginseng flower extract showed no mouse bone marrow micronucleus nor sperm abnormality effect. CONCLUSION：Under the present experimental conditions,notoginseng flower extract showed no mutagenic effect on mice.