与结肠癌转移倾向相关的细胞粘附基因的筛查

目的： 利用cDNA基因芯片研究已发生转移与未发生转移的原发性结肠癌基因表达谱的差异，筛选与结肠癌转移相关的细胞粘附基因。方法：分别提取未发生转移及已发生转移的原发性结肠癌组织mRNA，逆转录成cDNA探针，经Cy3和Cy5标记后与含1.6万余点的cDNA基因芯片杂交，用专用软件分析杂交信号强度，找出与细胞粘附相关的差异基因，并进行功能分析。结果：已发生转移与未发生转移的原发性结肠癌基因表达谱有显著差异，与细胞粘附相关的基因中，荧光强度比值小于0.2或大于5.0的差异基因共26个。上调基因15个，包括CD44、ICAM1、NCAM、HMGB1、SELL、CHRNA5、CEACAM6、VCAM1、HMGA2、PECAM1、CEACAM1、CD18、ITGAL、TGFB1、ICAM-3。下调基因11个，包括CD99、CADM1、CD82、F11R、SELE、CD226、TSLC1、PTB、CAR、JAM-2、PTEN。结论：结肠癌转移是由多个基因参与的复杂过程，细胞粘附基因在转移过程中起着非常重要的作用。

To screen cell adhesion genes related to metastasis in colon cancer

OBJECTIVE：To analyze the gene expression in colon cancer including non-metastasized and already metastasized using cDNA gene chip. To screen cell adhesion genes in colon cancer metastasis-associated genes. METHODS：The cDNAs reversely transcribed from the mRNA derived respectively from one non-metastasized and already metastasized were labeled with Cy3 and Cy5 prior to hybridization with the gene chip with 16k genes. The acquired was analyzed by software. Genes associated with cell adhesion and their function were analyzed. RESULTS：There were significant differences in gene expression profiles between non-metastasized and metastasized. When the fold change criteria were set to be≥5 or ≤-5 fold,26 genes were differentially expressed,15 genes were significantly up-regulated,including CD44,ICAM1,NCAM,HMGB1,SELL,CHRNA5,CEACAM6,VCAM1,HMGA2, PECAM1,CEACAM1,CD18,ITGAL,TGFB1,ICAM-3;and 11 were down-regulated with CD99, CADM1, CD82, F11R,SELE,CD226,TSLC1,PTB,CAR,JAM-2 and PTEN included. CONCLUSION：Colon cancer metastasis requires multiple genes to be involved. It is therefore important to use gene chip technology to screen cell adhesion among the genes associated with metastasis in colon cancer.