蒙药荜茇提取物荜茇宁的致畸试验研究

目的： 探讨蒙药荜茇提取物荜茇宁对大鼠的致畸作用。方法：将Wistar孕鼠75只随机分成5组，每组15只。受试组于妊娠第6～15天分别灌胃高 (500 mg/kg)、中 (100 mg/kg)、低 (20 mg/kg)剂量的荜茇宁混悬液，阴性对照组灌服等容量0.5%羧甲基纤维素钠水溶液，阳性对照组于妊娠第11天一次性腹腔注射环磷酰胺10 mg/kg。记录妊娠第0、3、7、10、13、16、20天孕鼠的体质量。于妊娠第20天处死，剖腹检查受孕情况及胎鼠外观和内脏、骨骼形态。结果：与阴性对照组相比，荜茇宁各剂量组对孕鼠外观，胎鼠外观、胎鼠生长指标和胎鼠顶骨、胸骨、肋骨等骨骼的骨化程度以及胎鼠主要脏器，均无明显影响 (P>0.05)。在实验过程中，500 mg/kg荜茇宁剂量组出现了2个吸收胎，但与阴性组相比，差异无统计学意义 (P>0.05)。结论：荜茇宁在本实验条件下，对孕鼠和胎鼠均无明显的胚胎毒性和致畸毒性。

Teratogenicity of the natural effective component piperlonguminine of Piper longum L.

OBJECTIVE：To study the teratogenicity of the natural effective component piperlonguminine Piper longum L. on normal Wistar pregnant rats. METHODS：Rats were randomly divided into 5 group. Stomach of the Wistar rat was irrigated with different doses of piperlonguminine suspension, high dosage 500 mg/kg, medium dosage 100 mg/kg, low dosage 20 mg/kg on the 6th to the 15th day during the gestation period. Stomach of the negative group was irrigated with the same volume CMC-Na solution. For the positive group,intraperitoneal cyclophosphamide 10 mg/kg was administered on the 11th day of gestation. Weights on the 0,3rd,7th,10th, 13th,16th,20th days were recorded. All were killed on the 20th day of gestation. The situation of the pregnancy, appearance of internal organs and bone morphology were examined. RESULTS：Compared to each dose group and negative group,the appearance and weight gain of pregnant rats, the appearance of the fetus, growth indicators and the degree of ossification of the occipital bone, parietal bone, sternum, ribs and other bones ; and the appearance of the liver, kidney, testis or uterus and other organs revealed no significant difference （P0.05）. During the experiment,2 fetuses were absorbed in the high dose group,but was insignificant when compared with the negative group （P0.05）. CONCLUSION：The lipid-lowering active ingredients of Mongolian drug piperlonguminine,under our experimental dose range, did not demonstrated obvious toxicity in pregnant mice and fetuses .