| 胃癌组织中S100A2基因的突变及杂合性缺失研究 |
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| 引用本文： | 赵晓春,刘迎福,贺修胜,禹正杨,唐振. 胃癌组织中S100A2基因的突变及杂合性缺失研究[J]. 中国现代医学杂志, 2012, 22(27): 19-22 |
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| 作者姓名： | 赵晓春 刘迎福 贺修胜 禹正杨 唐振 |
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| 作者单位： | 1. 南华大学附属第一医院肿瘤外科
2. 南华大学医学院肿瘤研究所,湖南衡阳,421001 |
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| 摘 要： | 目的 检测胃癌组织中S100A2基因的突变及杂合性缺失,从DNA水平探讨S100A2基因在胃癌发生发展过程中的作用.方法 采用PCR-SSCP和微卫星结合PCR-变性聚丙烯酰胺凝胶电泳技术,分析胃癌组织中S100A2基因的突变及缺失情况.结果 40例胃癌标本中,未发现S100A2基因缺失,亦未检测到S100A2基因第二外显子和第三外显子的突变.结论 S100A2基因在胃癌中的表达下调可能不是由S100A2基因杂合性缺失与突变所致.
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| 关 键 词： | 胃癌 S100A2基因 突变 杂合性缺失 |
Mutatioin,and deletion of S100A2 gene in gastric carcinoma |
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| ZHAO Xiao-chun,LIU Ying-fu,HE Xiu-sheng,YU Zheng-yang,TANG Zhen. Mutatioin,and deletion of S100A2 gene in gastric carcinoma[J]. China Journal of Modern Medicine, 2012, 22(27): 19-22 |
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| Authors: | ZHAO Xiao-chun LIU Ying-fu HE Xiu-sheng YU Zheng-yang TANG Zhen |
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| Affiliation: | 1(1.Department of Surgical Oncology,the First Affiliated Hospital of University of South China,Hengyang,Hunan 421001,P.R.China;2.Cancer Research Institute of Medical College, University of South China,Hengyang,Hunan 421001,P.R.China) |
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| Abstract: | 【Objective】 The mutation and LOH of S100A2 gene were detected,in order to clarify the role of S100A2 gene in the process of carcinogenesis and development in stomach.【Methods】 The mutation and deletion of S100A2 gene were detected with PCR-SSCP and denaturing PAGE gel electrophoresis in gastric carcinoma.【Results】 There were no mutation in the microsatellite DNA D1S3498,the exon 2 and exon 3 of S100A2 in gastric caicinoma.【Conclusion】 The down-regulation of S100A2 expression could not be related to the mutation and deletion of S100A2 gene in gastric carcinoma. |
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| Keywords: | gastric carcinoma S100A2 gene mutation LOH |
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