| 聚集性大肠埃希菌生物膜形成及毒力基因型研究 |
| |
| 引用本文： | 赵雪涛,沈小婷. 聚集性大肠埃希菌生物膜形成及毒力基因型研究[J]. 中国卫生检验杂志, 2012, 0(7): 1487-1490,1494 |
| |
| 作者姓名： | 赵雪涛 沈小婷 |
| |
| 作者单位： | 上海市徐汇区疾控中心微生物检验科 |
| |
| 摘 要： | 目的:通过肠道门诊标本检出的聚集性大肠埃希菌,研究其相关致病因子的毒力基因。方法:使用生物膜形成试验筛检聚集性大肠埃希菌,获得菌株后,进行转译激活因子基因,CVD432质粒基因,聚集性大肠埃希菌Ⅰ型鞭毛基因、Ⅱ型鞭毛基因、Ⅲ型鞭毛基因、Ⅳ菌毛基因,质粒编码毒素基因,聚集性大肠埃希菌耐热性肠毒素基因等各种基因检测,研究不同基因的分布和生物膜实验的相关性。结果:可以通过生物膜形成试验筛检聚集性大肠埃希菌,并根据毒力基因分型。结论:生物膜形成实验作为利用生物学特性筛检聚集性大肠埃希菌有一定的应用价值,聚集性大肠埃希菌的生物膜的形成与其各类毒力基因有不同程度的相关性。
|
| 关 键 词： | 聚集性大肠埃希菌 生物膜形成 毒力基因分型 |
Study on virulence genotypes and bio-film formation of Entero-aggregative Escherichia coli |
| |
| ZHAO Xue-tao,SHEN Xiao-ting. Study on virulence genotypes and bio-film formation of Entero-aggregative Escherichia coli[J]. Chinses Journal of Health Laboratory Technology, 2012, 0(7): 1487-1490,1494 |
| |
| Authors: | ZHAO Xue-tao SHEN Xiao-ting |
| |
| Affiliation: | (Department of Microbiology,Xuhui District Center for Diease Control and Prevention,Shanghai 200237,China) |
| |
| Abstract: | Objective:To study the association of Enteroaggregative Escherichia coli(EAggEC) virulence genes and bio-film formation.Methods: Biofilm formation test was used to screen EAgg EC and PCR was used to detect Agg R,AAT(CVD432),agga(Aggregative fimbria I),aafa(AAF/II),agg3a(AAF/III),pilS(Type IV pilus),pet(Plasmid-encoded toxin),eastA(EAggEC heat-stable toxin) genes and the relation between bio-film formation and virulence genes was analyzed.Results: Bio-film formation test can be used for screening of EAgg EC,which can be classified by virulence genotyping.Conclusion: Biofilm formation test is useful to screen Enteroaggregative Escherichia coli(EAggEC),and it has correlation with every virulence gene to varying degrees. |
| |
| Keywords: | Enteroaggregative Escherichia coli Bio-film formation Virulence genotype |
| 本文献已被 CNKI 等数据库收录！ |
|
