环氧化酶-2、血管内皮生长因子在大肠癌中的表达及意义

目的:探讨环氧化酶-2、血管内皮生长因子在大肠腺癌中的表达及临床意义,并分析两者在大肠腺癌癌变过程中的作用。方法:运用免疫组织化学SP法检测大肠腺癌53例、大肠腺瘤12例和正常大肠粘膜10例中环氧化酶-2、血管内皮生长因子的表达情况,并分析其与大肠腺癌临床病理特征之间的关系,以及两者之间的关系。结果:(1)在大肠腺癌、大肠腺瘤与正常大肠粘膜中,环氧化酶-2的阳性表达率分别为83.0%(44/53)、83.3%(10/12)和20.0%(2/10);血管内皮生长因子为86.8%(46/53)、66.7%(8/12)和30.0%(3/10)。环氧化酶-2的表达在大肠腺癌与正常大肠粘膜、大肠腺瘤与正常大肠粘膜之间差异有统计学意义(P<0.01,P<0.01)。血管内皮生长因子的表达在大肠腺癌与正常大肠粘膜之间差异有统计学意义(P<0.01)。环氧化酶-2与血管内皮生长因子在大肠腺癌中的表达均呈显著正相关(均为P<0.05)。(2)大肠腺癌53例中环氧化酶-2的表达与肿瘤分化程度、浸润深度关系密切(P<0.05,P<0.05)。大肠腺癌53例中血管内皮生长因子的表达与肿瘤分化程度、浸润深度关系密切(P<0.05,P<0.01)。结论:环氧化酶-2与血管内皮生长因子在大肠癌中的表达呈正相关,提示环氧化酶-2可能通过某种机制促进血管内皮生长因子的表达,共同参与大肠腺癌的发生发展。

The expression and significance of COX-2 and VEGF in colorectal adenocarcinoma

Objective:To explore the expression and clinical effects between cyclooxygenase2(COX-2)and vascular endothelial growth factor(VEGF)protein in colorectal adenocarcinoma,and detect the roles between these factors in the development of colorectal adenocarcinoma.Methods:Immunohistochemical SP staining method was used to detect the expression of COX-2 and VEGF in 53 cases of colorectal adenocarcinoma,12 cases of colorectal adenoma and 10 cases of normal colorectal mucosa.And the relationship between these factors and clinicopathologic characteristics and the relationship of the factors were analyzed.Results:(1)In clinical samples the positive rates of COX-2 in colorectal adenocarcinoma,colorectal adenoma and normal colorectal mucosa were 83.0%(44/53),83.3%(10/12) and 20%(2/10),respectively.Compared with the positive rate of normal colorectal mucosa,the positive rates in every other group showed significant difference(P<0.01,P<0.01).The positive rates of VEGF in colorectal adenocarcinoma,colorectal adenoma and normal colorectal mucosa were 86.8%(46/53),66.7%(8/12) and 30.0%(3/10),respectively.Its positive rate showed significant difference(P<0.01) between colorectal adenocarcinoma and normal colorectal mucosa.Spearman correlation analysis showed that there was a significant positive correlatin between the expression of COX-2 and VEGF,respectively(P<0.05).(2)The expression of COX-2 in 53 cases of colorectal adenocarcinoma was significantly correlated with the degree of differentiation and the depth of infiltration(P<0.05,P<0.05).The expression of VEGF in 53 cases of colorectal adenocarcinoma was significantly correlated with the degree of differentiation and the depth of infiltration(P<0.05,P<0.01).Conclusions:A significance positive correlation between COX-2 and VEGF suggests COX-2 may up-regulate the expression of VEGF to promote the development and progression of colorectal adenocarcinoma.