脑血管痉挛中差异表达蛋白质的实验研究

目的 建立兔脑血管痉挛(CVS)基底动脉和正常兔基底动脉双向凝胶电泳图谱,鉴定差异表达蛋白,从中发现有意义的CVS相关标志物.方法 采用双向凝胶电泳(2-DE)分离痉挛基底动脉和正常基底动脉总蛋白,银染显色,通过imagemaster5.0软件分析,从中选取差异表达蛋白质点,应用基质辅助激光解析飞行时间串联质谱(MALDI-TOF/TOF)鉴定差异表达的蛋白质.结果 获得重复性和分辨率较好的兔基底动脉双向凝胶电泳图谱;发现49个差异表达点,其中35个点得到鉴定,在CVS中高表达的有24个,其余11个在CVS中呈低表达.结论 建立了痉挛基底动脉和正常基底动脉双向凝胶电泳图谱,并应用质谱技术鉴定了35个差异表达点,这些差异表达蛋白质可能与CVS的发生相关.

Experimental study of differential expression protein on cerebral vasospasm in rabbits

Objective To establish two-dimensional electrophoresis (2-DE) profiles of basilar arteries and paired normal basilar arteries of cerebral vasospasm in rabbit to identify differential expression proteins in order to find out candidates as cerebral vasospasm-related proteins. Methods 2-DE was applied to separate the total proteins of cerebral vasospasm basilar arteries and paired normal basilar arteries.After staining and analyzing by imagemaster 5.0 software. The differential proteins were identified by MALDI-TOF/TOF. Results 2-DE profiles with high resolution and reproducibility was obtained. Forty-nine protein spots were found as differentially-expressed proteins, in which 35 protein spots were successfully identified. Among the identified proteins, there were 24 over-expressed and 11 under-expressed proteins in spasm basilar arteries as compared with normal basilar arteries. Conclusions Established 2-DE patterns of rabbit spasm basilar arteries and paired normal basilar arteries and 35 differentially expressed proteins spots are identified, and which may participate in cerebral vasospasm.