| 荧光定量PCR检测乙型肝炎患者血清中HBV DNA的临床意义 |
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| 引用本文： | 张阳根,张横,徐忠玉,张德慧.荧光定量PCR检测乙型肝炎患者血清中HBV DNA的临床意义[J].医学研究生学报,2001,14(Z1):13-15. |
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| 作者姓名： | 张阳根 张横 徐忠玉 张德慧 |
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| 作者单位： | 解放军第一七五医院检验科,福建漳州,363000 |
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| 摘 要： | 目的检测乙型肝炎患者血清中乙型肝炎病毒(HBV)DNA的含量，了解其与免疫学指标的关系。方法采用荧光定量PCR技术和ELISA法，分别检测287例乙型肝炎患者血清中HBVDNA含量和免疫学指标。结果287例乙型肝炎患者血清中检出HBVDNA199例，阳性率为69.34%(199/287)；144例HBsAg、HBeAg、抗HBc三项阳性的血清，其血清中HBVDNA也全部阳性；而HBsAg、抗HBe、抗HBc三项阳性和HBsAg、抗HBc二项阳性的标本，HBVDNA的检出率分别38.9%(42/108)和37.1%(13/35)。结论乙型肝炎患者血清中HBVDNA的拷贝数与HBeAg密切相关，但系统转换不能说明HBV停止复制，而只是复制水平的降低而已。
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| 关 键 词： | 荧光定量 PCR技术 HBVDNA 免疫学指标 |
| 文章编号： | 1008-8199(2001)增刊-0013-02 |
| 修稿时间： | 2000年12月20 |
Determination of serum HBV DNA by fluorescence quantitative PCR in patients with hepatitis B and its clinical application |
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| ZHANG Yang-gen,ZHANG Heng,XU Zhong-yu,ZHANG De-hui.Determination of serum HBV DNA by fluorescence quantitative PCR in patients with hepatitis B and its clinical application[J].Bulletin of Medical Postgraduate,2001,14(Z1):13-15. |
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| Authors: | ZHANG Yang-gen ZHANG Heng XU Zhong-yu ZHANG De-hui |
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| Abstract: | Objectives:To study the relationship between the amount of serum HBV DNA and immune indexes by fluorescence quantitative PCR(FQ PCR) technique. Methods:FQ PCR technique and ELISA method were used to detect HBV DNA contents and immune indexes in 287 patients with hepatitis B. Results: HBV DNA were found in 199 cases from 287 patients with hepatitis B, the positive rate was 69.3%(199/258).In 144 HBsAg +HBeAg +/HBcAb + samples, FQ PCR results were positive, but in HBsAG +/HBeAb +/HBcAb + and HBsAg +/HBcAb + samples, the positive rate of FQ PCR were 38.9%(42/108) and 37.1%(13/35) respectively. Conclusions:The average levels of serum HBV DNA was closely correlated with HBeAg in hepatitis B patients. Changes of the system indicated reduction but not cessation of amplification. |
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| Keywords: | Fluoresence quantitative PCR HBV DNA Immune indexes |
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